Archives of Virology

, Volume 140, Issue 11, pp 2067–2073

Expression and analysis of the NS2 protein of influenza A virus

  • A. C. Ward
  • L. A. Castelli
  • A. C. Lucantoni
  • J. F. White
  • A. A. Azad
  • I. G. Macreadie
Brief Report

DOI: 10.1007/BF01322693

Cite this article as:
Ward, A.C., Castelli, L.A., Lucantoni, A.C. et al. Archives of Virology (1995) 140: 2067. doi:10.1007/BF01322693

Summary

Influenza NS2 protein was expressed inSaccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • A. C. Ward
    • 1
    • 2
  • L. A. Castelli
    • 1
  • A. C. Lucantoni
    • 1
  • J. F. White
    • 3
  • A. A. Azad
    • 1
  • I. G. Macreadie
    • 1
  1. 1.Biomolecular Research InstituteParkville
  2. 2.Russell Grimwade School of BiochemistryUniversity of MelbourneParkville
  3. 3.CSIRO Division of Biomolecular EngineeringParkvilleAustralia