Expression and analysis of the NS2 protein of influenza A virus
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- Ward, A.C., Castelli, L.A., Lucantoni, A.C. et al. Archives of Virology (1995) 140: 2067. doi:10.1007/BF01322693
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Influenza NS2 protein was expressed inSaccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.