Archives of Virology

, Volume 116, Issue 1, pp 159–173

HT-29 cells: a new substrate for rotavirus growth

  • F. Superti
  • A. Tinari
  • L. Baldassarri
  • G. Donelli
Original Papers

DOI: 10.1007/BF01319239

Cite this article as:
Superti, F., Tinari, A., Baldassarri, L. et al. Archives of Virology (1991) 116: 159. doi:10.1007/BF01319239

Summary

Susceptibilities of a continuous rhesus monkey kidney cell line (MA-104) and that of a human colon carcinoma cell line (HT-29) to infection by different human and animal rotavirus strains were compared.

HT-29 cells appeared to be more sensitive to human rotavirus infection than MA-104 cells, whereas the latter cell line was more susceptible to animal rotavirus replication. The greater sensitivity to human rotavirus infection of HT-29 cells was confirmed by the successful, direct isolation of these viruses from faecal specimens. Human rotavirus infection of HT-29 cells was also followed by transmission electron microscopy. In ultra-thin sections, unenveloped particles of rotaviruses, representing infectious mature virions, were observed in large number. Moreover, many “double-shelled” particles were detected in negative-stained supernatants from infected cultures. Scanning electron microscopy of uninfected HT-29 cells showed that in the presence of Ca+ +, required for rotavirus growth, they are able to express some of the features of mature intestinal cells.

In view of these results, HT-29 cells appear to be a useful in vitro model for the study of rotavirus infection.

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • F. Superti
    • 1
  • A. Tinari
    • 1
  • L. Baldassarri
    • 1
  • G. Donelli
    • 1
  1. 1.Laboratorio di UltrastruttureIstituto Superiore di SanitàRomaItaly