Archives of Virology

, Volume 116, Issue 1, pp 69–80

NS2 protein of influenza virus is found in purified virus and phosphorylated in infected cells

  • J. C. Richardson
  • R. K. Akkina
Original Papers

DOI: 10.1007/BF01319232

Cite this article as:
Richardson, J.C. & Akkina, R.K. Archives of Virology (1991) 116: 69. doi:10.1007/BF01319232


Purified viral preparations of influenza A virus were examined for the presence of NS2 protein hitherto considered as a viral nonstructural protein that is present only in infected cells. Analysis of purified virus by radioimmunoprecipitation with monospecific antisera to NS2 revealed its presence in the virus particle suggesting that it is a viral structural protein. NS2 protein was also shown to be phosphorylated in infected cells in this study. This brings the number of influenza virus phosphoproteins to three which include NP, NS1, and NS2. These observations raise important questions about the role of NS2 in the replication of influenza virus.

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. C. Richardson
    • 1
  • R. K. Akkina
    • 1
  1. 1.Department of Microbiology, College of Veterinary Medicine and Biomedical SciencesColorado State UniversityFort CollinsUSA
  2. 2.Department of MicrobiologyMount Sinai School of MedicineNew YorkUSA