Original Articles

Digestive Diseases and Sciences

, Volume 38, Issue 3, pp 529-537

First online:

Histochemical study of colonic cancer in experimental colitis of rats

  • Takaji TamaruAffiliated withFirst Department of Internal Medicine, Hiroshima University School of MedicineDepartment of Medicine, Veterans Affairs Medical Center and University of Colorado School of MedicineFirst Department of Pathology, Hiroshima University School of Medicine
  • , Hirofumi KobayashiAffiliated withFirst Department of Internal Medicine, Hiroshima University School of MedicineDepartment of Medicine, Veterans Affairs Medical Center and University of Colorado School of MedicineFirst Department of Pathology, Hiroshima University School of Medicine
  • , Shinya KishimotoAffiliated withFirst Department of Internal Medicine, Hiroshima University School of MedicineDepartment of Medicine, Veterans Affairs Medical Center and University of Colorado School of MedicineFirst Department of Pathology, Hiroshima University School of Medicine
  • , Goro KajiyamaAffiliated withFirst Department of Internal Medicine, Hiroshima University School of MedicineDepartment of Medicine, Veterans Affairs Medical Center and University of Colorado School of MedicineFirst Department of Pathology, Hiroshima University School of Medicine
  • , Fumio ShimamotoAffiliated withFirst Department of Internal Medicine, Hiroshima University School of MedicineDepartment of Medicine, Veterans Affairs Medical Center and University of Colorado School of MedicineFirst Department of Pathology, Hiroshima University School of Medicine
  • , William R. BrownAffiliated withFirst Department of Internal Medicine, Hiroshima University School of MedicineDepartment of Medicine, Veterans Affairs Medical Center and University of Colorado School of MedicineFirst Department of Pathology, Hiroshima University School of Medicine

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Abstract

A reliable test for premalignant lesions in the development of colonic cancer in chronic ulcerative colitis has been needed. Thus, we designed this cytochemical study, using a model of experimental colitis and colonic tumors induced in Wistar male rats by the feeding of dextran sulfate sodium. The colitis had histologic similarities to ulcerative colitis in man. The percent frequency of polypoid lesions (dysplasia or dysplasia with carcinomain situ) in the cecum and ascending colon was about 25% at three months and 90% at six months of dextran sulfate feeding. The cytochemical findings by high-iron diamine-Alcian blue staining andUlex europeus agglutinin binding were chronologically paralledled by histological changes in the colonic mucosa, and the binding pattern of peanut agglutinin was not different between normal and dextran-treated animals. Moreover, some cytochemical changes that occurred during the inflammatory responses were not present in dysplastic or malignant lesions. Thus, the histochemical tests were not useful for detecting of premalignant lesions earlier than by conventional histology. Nevertheless, the dextran sulfate model of colitis in the rat appears suitable for study of cancer development in ulcerative colitis.

Key words

ulcerative colitis dysplasia colon cancer high-iron diamine-Alcian blue staining Ulex europeus agglutinin dextran sulfate sodium