Archives of Virology

, Volume 105, Issue 1, pp 39–53

Variation of the nucleotide and encoded amino acid sequences of the envelope gene from eight dengue-2 viruses

  • J. Blok
  • S. Samuel
  • A. J. Gibbs
  • U. T. Vitarana
Original Papers

DOI: 10.1007/BF01311115

Cite this article as:
Blok, J., Samuel, S., Gibbs, A.J. et al. Archives of Virology (1989) 105: 39. doi:10.1007/BF01311115

Summary

The nucleotide sequences of the envelope genes from five Thai and three Sri Lankan dengue-2 viruses were determined by sequencing the viral RNA using synthetic oligonucleotide primers. The results were compared with the four published dengue-2 envelope sequences to obtain a classification of these viruses, which showed that the Thai isolates could be divided into two separate groups while the Sri Lankan isolates were distinct. There was no correlation between disease severity and envelope protein sequence, or between year of isolation and sequence. No particular amino acid changes were associated with virulence or a change in hydrophilic region which could perhaps act as an epitope.

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. Blok
    • 1
  • S. Samuel
    • 1
  • A. J. Gibbs
    • 2
  • U. T. Vitarana
    • 3
  1. 1.Sir Albert Sakzewski Virus Research LaboratoryRoyal Children's HospitalBrisbane
  2. 2.Research School of Biological SciencesAustralian National UniversityCanberraAustralia
  3. 3.Medical Research InstituteColomboSri Lanka
  4. 4.Arbovirus Research Unit, Department of Medical Microbiology, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia