Archives of Virology

, Volume 139, Issue 3, pp 351–363

Characterization of three different transgenic mouse lines that carry human poliovirus receptor gene—influence of the transgene expression on pathogenesis

  • S. Koike
  • C. Taya
  • J. Aoki
  • Y. Matsuda
  • I. Ise
  • H. Takeda
  • T. Matsuzaki
  • H. Amanuma
  • H. Yonekawa
  • A. Nomoto
Original Papers

DOI: 10.1007/BF01310797

Cite this article as:
Koike, S., Taya, C., Aoki, J. et al. Archives of Virology (1994) 139: 351. doi:10.1007/BF01310797

Summary

Three transgenic mouse lines, ICR-PVRTg1, ICR-PVRTg5, and ICR-PVRTg21, which are susceptible to poliovirus, have been established by introducing the human gene for poliovirus receptor (PVR) into the genome of mouse strain ICR. Genetic characterizations of the PVR gene were carried out on these mouse lines to define the approximate copy number, insertion site, and expression of the transgene in the central nervous system (CNS). The transgene was integrated in the chromosome 4, 12, and 13 of ICR-PVRTg1, ICR-PVRTg5 and ICR-PVRTg21 mice, respectively, and was stably transmitted to progeny mice. ICR-PVRTg1 appeared to have the most abundant copy numbers of the transgene and showed the highest level of PVR mRNA and membrane associated PVR protein in the CNS among the three mouse lines. Those in ICR-PVRTg21 and ICR-PVRTg5 were at intermediate and lowest levels, respectively. In the CNS, PVR mRNA was detected at high levels only in neurons of the spinal cord and brain stem where poliovirus can replicate, suggesting that the PVR mRNA expression confers cell specificity to poliovirus in the CNS. ICR-PVRTg1 and ICR-PVRTg5 showed the highest and the lowest sensitivity to poliovirus, respectively, whereas ICR-PVRTg21 was in-between. These results may suggest that poliovirus sensitivity of the mice is attributed to relative levels of PVR expression.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • S. Koike
    • 1
  • C. Taya
    • 2
  • J. Aoki
    • 1
  • Y. Matsuda
    • 3
  • I. Ise
    • 1
  • H. Takeda
    • 4
  • T. Matsuzaki
    • 4
  • H. Amanuma
    • 4
  • H. Yonekawa
    • 2
  • A. Nomoto
    • 1
    • 5
  1. 1.Department of MicrobiologyThe Tokyo Metropolitan Institute of Medical ScienceTokyo
  2. 2.Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical ScienceTokyo
  3. 3.Department of GeneticsNational Institute of Radiological SciencesChiba
  4. 4.The Institute of Physical and Chemical ResearchIbaraki
  5. 5.Department of Microbiology, The Institute of Medical ScienceThe University of TokyoTokyoJapan
  6. 6.Institut PasteurParisFrance

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