Archives of Virology

, Volume 115, Issue 1, pp 37–46

Hantavirus strains isolated from rodentia and insectivora in rural China differentiated by polymerase chain reaction assay

Authors

  • Y. W. Tang
    • Department of Epidemiology, School of Public HealthShanghai Medical University
    • Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease ControlPublic Health Service, U.S. Department of Health and Human Services
    • Department of Infectious Diseases, First Affiliated HospitalZhejiang Medical University
  • S. L. Ruo
    • Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease ControlPublic Health Service, U.S. Department of Health and Human Services
    • Department of Infectious Diseases, First Affiliated HospitalZhejiang Medical University
  • A. Sanchez
    • Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease ControlPublic Health Service, U.S. Department of Health and Human Services
    • Department of Infectious Diseases, First Affiliated HospitalZhejiang Medical University
  • Susan P. Fisher-Hoch
    • Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease ControlPublic Health Service, U.S. Department of Health and Human Services
    • Department of Infectious Diseases, First Affiliated HospitalZhejiang Medical University
  • J. B. McCormick
    • Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease ControlPublic Health Service, U.S. Department of Health and Human Services
    • Department of Infectious Diseases, First Affiliated HospitalZhejiang Medical University
  • Z. Y. Xu
    • Department of Epidemiology, School of Public HealthShanghai Medical University
    • Department of Infectious Diseases, First Affiliated HospitalZhejiang Medical University
Original Papers

DOI: 10.1007/BF01310621

Cite this article as:
Tang, Y.W., Ruo, S.L., Sanchez, A. et al. Archives of Virology (1990) 115: 37. doi:10.1007/BF01310621

Summary

Polymerase chain reaction (PCR) assay and other techniques were applied to differentiate Hantavirus strains isolated from different animal hosts and geographic regions in China. Two groups of related strains, Hantaan and Seoul, have been classified by cross-neutralization, radioimmunoprecipitation (RIP), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assays. The molecular weights of glycoprotein 1 (G1) of Hantaan and Seoul viruses were 72k and 80k, whereas those of the nucleocapsid (N) and glycoprotein 2 (G2) remained the same, respectively. The PCR assay was used to differentiate these isolates using synthetic oligonucleotide primers selected from various regions of the M genome of 76118 and R22 strains. 76118-specific primers amplified only the RNAs extracted from Hantaan strains while R22-specific primers, the RNAs from Seoul strains. The PCR results for classification are consistent with those obtained by cross-neutralization, RIP and SDS-PAGE assays.

Copyright information

© Springer-Verlag 1990