Archives of Virology

, Volume 132, Issue 1, pp 145–159

PCR detection of the sheep-associated agent of malignant catarrhal fever

  • S. I. F. Baxter
  • I. Pow
  • A. Bridgen
  • H. W. Reid
Original Papers

DOI: 10.1007/BF01309849

Cite this article as:
Baxter, S.I.F., Pow, I., Bridgen, A. et al. Archives of Virology (1993) 132: 145. doi:10.1007/BF01309849

Summary

From a genomic library previously constructed from a lymphoblastoid cell line (LCL) propagated from a bovine case of sheep-associated malignant catarrhal fever (SA-MCF), caused by ovine herpesvirus-2 (OHV-2), several OHV-2 clones were identified and characterised by hybridisation using probes from the unique region of the Alcelaphine herpesvirus-1 (AVH-1) genome. Nucleotide sequence from one clone was generated and the predicted amino acid sequence was found to contain regions of homology with the 140 and 160 kDa tegument proteins of Epstein-Barr virus and herpesvirus saimiri respectively. Oligonucleotide primers were constructed and a polymerase chain reaction (PCR) test was developed for the detection of OHV-2 viral DNA. Amplified product was identified by restriction withRsa I andBmy I. The primers were highly specific for OHV-2 DNA with a limit of detection of 6.4 pg of genomic DNA derived from the parent LCL. This was estimated to correspond to one diploid bovine cell. The PCR was successfully applied to detect OHV-2 DNA in peripheral blood leucocytes (pbl) from clinical cases of SA-MCF and normal sheep.

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • S. I. F. Baxter
    • 1
  • I. Pow
    • 1
  • A. Bridgen
    • 1
  • H. W. Reid
    • 1
  1. 1.Moredun Research InstituteEdinburghU.K.
  2. 2.Institute of VirologyUniversity of GlasgowU.K.