Archives of Virology

, Volume 137, Issue 3, pp 303–313

Flavivirus West Nile (Sarafend) egress at the plasma membrane

  • M. L. Ng
  • J. Howe
  • V. Sreenivasan
  • J. J. L. Mulders
Original Papers

DOI: 10.1007/BF01309477

Cite this article as:
Ng, M.L., Howe, J., Sreenivasan, V. et al. Archives of Virology (1994) 137: 303. doi:10.1007/BF01309477

Summary

West Nile (Sarafend) virus was distinctly observed to bud from the plasma membrane rather than mature intracellularly. This has been observed with transmission electron microscopy. Using conventional scanning electron microscopy, budding at the plasma membrane especially at the filopodia was clearly illustrated. Immunogold labelling against the virus envelope protein was also performed to confirm this mode of exit. The gold particles were observed to be located at the sites where virus budding was seen under the field emission scanning electron microscope.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. L. Ng
    • 1
  • J. Howe
    • 1
  • V. Sreenivasan
    • 1
  • J. J. L. Mulders
    • 2
  1. 1.Department of MicrobiologyNational University of SingaporeSingaporeRepublic of Singapore
  2. 2.Application LaboratoryPhilipsEndhovenThe Netherlands