Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection
- Cite this article as:
- Agapov, E.V., Razumov, I.A., Frolov, I.V. et al. Archives of Virology (1994) 139: 173. doi:10.1007/BF01309462
Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.