Archives of Virology

, Volume 139, Issue 1, pp 173–181

Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection

Authors

  • E. V. Agapov
    • Institute of Molecular BiologyState Research Center of Virology and Biotechnology "Vector"
  • I. A. Razumov
    • Institute of Molecular BiologyState Research Center of Virology and Biotechnology "Vector"
  • I. V. Frolov
    • Institute of Molecular BiologyState Research Center of Virology and Biotechnology "Vector"
  • A. A. Kolykhalov
    • Institute of Molecular BiologyState Research Center of Virology and Biotechnology "Vector"
  • S. V. Netesov
    • Institute of Molecular BiologyState Research Center of Virology and Biotechnology "Vector"
  • V. B. Loktev
    • Institute of Molecular BiologyState Research Center of Virology and Biotechnology "Vector"
Brief Report

DOI: 10.1007/BF01309462

Cite this article as:
Agapov, E.V., Razumov, I.A., Frolov, I.V. et al. Archives of Virology (1994) 139: 173. doi:10.1007/BF01309462

Summary

Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.

Copyright information

© Springer-Verlag 1994