Digestive Diseases and Sciences

, Volume 37, Issue 6, pp 818–826

Tumor necrosis factor-α, interleukin-1β, and interleukin-6 expression in inflammatory bowel disease

  • Chris Stevens
  • Gerd Walz
  • Chander Singaram
  • Mark L. Lipman
  • Bernd Zanker
  • Aldo Muggia
  • Donald Antonioli
  • Mark A. Peppercorn
  • Terry B. Strom
Original Articles

DOI: 10.1007/BF01300378

Cite this article as:
Stevens, C., Walz, G., Singaram, C. et al. Digest Dis Sci (1992) 37: 818. doi:10.1007/BF01300378

Abstract

The etiology of ulcerative colitis (UC) and Crohn's disease (CD) remains enigmatic. Infiltrating intestinal macrophages are capable of producing the proinflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). We investigated the presence of IL-6, TNF-α and IL-1β mRNA transcripts in inflammatory bowel disease (IBD), normal, and other inflammatory intestinal specimens utilizing the polymerase chain reaction (PCR). TNF-α mRNA levels did not vary between inflammatory bowel disease and control specimens. IL-1β mRNA levels were highest in active UC and noninflammatory bowel disease inflammatory specimens while IL-6 mRNA levels were highest in active IBD specimens. Infiltrating T cells, macrophages, and B cells were identified as sources of IL-6 protein in inflammatory bowel disease specimens by immunofluorescent staining. IL-6 transcripts were elevated only in active inflammatory bowel disease specimens, suggesting that IL-6-mediated immune processes are ongoing in the inflammatory mucosal environment of CD and UC.

Key words

Crohn's diseaseulcerative colitispolymerase chain reactioninterleukin-6interleukin-1tumor necrosis factor

Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Chris Stevens
    • 1
  • Gerd Walz
    • 1
  • Chander Singaram
    • 1
  • Mark L. Lipman
    • 1
  • Bernd Zanker
    • 1
  • Aldo Muggia
    • 1
  • Donald Antonioli
    • 1
  • Mark A. Peppercorn
    • 1
  • Terry B. Strom
    • 1
  1. 1.Department of Medicine Center for Inflammatory Bowel DiseaseBeth Israel Hospital and Harvard Medical SchoolBoston
  2. 2.Departments of Gastroenterology and Clinical ImmunologyBeth Israel HospitalBoston