Digestive Diseases and Sciences

, Volume 32, Issue 11, pp 1281–1289

Modulation of collagen synthesis by fat-storing cells, isolated from CCl4-or vitamin A-treated rats

  • Yasushi Shiratori
  • Takafumi Ichida
  • Albert Geerts
  • Eddie Wisse
Original Articles

DOI: 10.1007/BF01296379

Cite this article as:
Shiratori, Y., Ichida, T., Geerts, A. et al. Digest Dis Sci (1987) 32: 1281. doi:10.1007/BF01296379

Abstract

In an attempt to elucidate the role of fat-storing cells (FSCs) in liver fibrosis, we investigated the collagen synthesis by FSCs freshly isolated from rats treated with CCl4, with vitamin A, and from untreated rats. FSCs from CCl4-treated rats contained a small number of lipid droplets and an abundant rough endoplasmic reticulum (RER), while those from vitamin A-treated rats showed numerous large lipid droplets and scanty RER. The population doubling times of FSCs isolated from normal, CCl4-treated, and vitamin A-treated rats were 38±4.3, 24±2.5, and 48±6.3 hr, respectively. The rate of collagen synthesis by FSCs from CCl4-treated rats was four- to sixfold enhanced, while collagen synthesis by FSCs from vitamin A-treated rats was suppressed. The ratio of collagen type I to type III produced by FSCs from CCl4 rats was enhanced as compared with control rats (94.7:5.3 vs 87.6:12.4). Therefore, FSCs can be considered to play an important role in the pathogenesis of liver fibrosis.

Key Words

fat-storing cells collagen synthesis vitamin A CCl4 liver fibrosis 

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Yasushi Shiratori
    • 1
    • 2
  • Takafumi Ichida
    • 1
    • 2
  • Albert Geerts
    • 1
    • 2
  • Eddie Wisse
    • 1
    • 2
  1. 1.Laboratory for Cell Biology and Histology, Faculty of Medicine and PharmacyFree University of Brussels (V.U.B.)Brussels-JetteBelgium
  2. 2.2nd Department of Internal Medicine, Faculty, of MedicineUniversity of TokyoTokyoJapan

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