Article

Protoplasma

, Volume 198, Issue 3, pp 210-217

Nuclear magnetic resonance microscopy ofAncistrocladus heyneanus

  • M. MeiningerAffiliated withLehrstuhl für Experimentelle Physik V, Universität Würzburg
  • , R. StowasserAffiliated withLehrstuhl für Organische Chemie I, Universität Würzburg
  • , P. M. JakobAffiliated withLehrstuhl für Experimentelle Physik V, Universität Würzburg
  • , H. SchneiderAffiliated withLehrstuhl für Biotechnologie, Universität Würzburg
  • , D. KopplerAffiliated withLehrstuhl für Organische Chemie I, Universität Würzburg
  • , G. BringmannAffiliated withLehrstuhl für Organische Chemie I, Universität Würzburg
  • , U. ZimmermannAffiliated withLehrstuhl für Biotechnologie, Universität Würzburg
  • , A. HaaseAffiliated withLehrstuhl für Experimentelle Physik V, Universität Würzburg

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Summary

The tropical lianaAncistrocladus heyneanus, which is known for its biologically active naphthylisoquinoline alkaloids, has been studied by nuclear magnetic resonance (NMR) microscopy for the first time. The spatial resolution of the cross-sectional NMR images was of the order of 20 μm. Quantitative NMR relaxation time images of the root and the shoot show great contrast between different tissue regions. In addition, we observed the regional distribution of chemical compounds inAncistrocladus heyneanus by chemical-shift NMR microscopy. The NMR imaging results were compared with light and fluorescence microscopic images and reveal the excellent tissue characterization using NMR technology.

Keywords

Nuclear magnetic resonance micro-imaging Chemical-shift imaging Fluorescence microscopy Ancistrocladus heyneanus Ancistrocladaceae Naphthylisoquinoline alkaloids