Rapid watet uptake and structural changes in imbibing seed tissues
- M. S. Buttrose
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Studies on ultrastructure in tissues from air-dry seeds normally involve fixation in aqueous media. Recent freeze-etch studies suggest that water is taken up extremely rapidly by these tissues with attendant structural change. Measures have now been made of water uptake by small (<400μg) pieces ofHordeum vulgare scutellum,Pisum sativum cotyledon and radicle, andHelianthus annuus cotyledon. Measurable water uptake occurred within 10 seconds, and scutellum was already saturated (160% of initial air-dry weight) within 2 minutes. Tissues re-dried almost as rapidly. Barley scutellum was investigated by freezeetching. Air-dry tissue, frozen direct in liquid nitrogen or surrounded by 100% glycerol, had convoluted cell walls, organelles with indented margins, and polyhedral lipid bodies which were in such close mutual proximity as to nearly form closed lipid sheets over plasmalemma and protein bodies. Within 8 seconds of exposing to water, cell walls had straightened and organelles and lipid bodies rounded out. It is likely that many descriptions of structure in “air-dry” seeds have been of this hydrated structure.
- Branton, D., 1969: Membrane structure. Ann. Rev. Plant Physiol.20, 209–238.
- Hallam, N. D., B. E. Roberts, andD. J. Osborne, 1972: Embryogenesis and germination in rye (Secale cereale L.). II. Biochemical and fine structural changes during germination. Planta105, 293–309.
- Johnson, R. P. C., 1968: Microfilaments in pores between frozen-etched sieve elements. Planta81, 314–332.
- Marinos, N. G., andD. N. Fife, 1972: Ultrastructural changes in wheat embryos during a “presowing drought hardening” treatment. Protoplasma74, 381–396.
- Moor, H., K. Mühlethaler, H. Waldner, andA. Frey-Wyssling, 1961: A new freezing ultramicrotome. J. biophys. biochem. Cytol.10, 1–13.
- Nieuwdorp, P. J., 1963: Electron microscopic structure of the epithelial cells of the scutellum of barley. The structure of the epithelial cells before germination. Acta Bot. Neerl.12, 295–301.
- Paulson, R. E., andL. M. Srivastava, 1968: The fine structure of the embryo ofLactuca sativa. I. Dry embryo. Canad. J. Bot.46, 1437–1446.
- Perner, E., 1965: Elektronenmikroskopische Untersuchungen an Zellen von Embryonen im Zustand völliger Samenruhe. I. Die zelluläre Strukturordnung in der Radicula lufttrockener Samen vonPisum sativum. Planta65, 334–357.
- Swift, J. G., andM. S. Buttrose, 1972 a: Freeze-etch studies of protein bodies in wheat scutellum. J. Ultrastruct. Res.40, 378–390.
- Swift, J. G., andM. S. Buttrose, 1972 b: Lipid layers, protein bodies, and plastids in freezeetched pea cotyledons. Planta109, 61–72.
- —, andT. P. O'Brien, 1972: The fine structure of the wheat scutellum before germination. Aust. J. Biol. Sci.25, 9–22.
- Yatsu, L. Y., 1965: The ultrastructure of cotyledonary tissue fromGossypium birsutum L. seeds. J. Cell Biol.25, 193–199.
- Rapid watet uptake and structural changes in imbibing seed tissues
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- M. S. Buttrose (1)
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- 1. Division of Horticultural Research, CSIRO, Adelaide, South Australia