Protoplasma

, Volume 200, Issue 3, pp 128–145

Mitotic cyclin distribution during maize cell division: Implications for the sequence diversity and function of cyclins in plants

Authors

  • Miriam Mews
    • Plant Cell Biology Group, Research School of Biological SciencesAustralian National University
    • Botanisches Institut der Universität Bonn
  • Frank J. Sek
    • Plant Cell Biology Group, Research School of Biological SciencesAustralian National University
  • Robert Moore
    • Cooperative Research Centre for Plant ScienceAustralian National University
  • Dieter Volkmann
    • Botanisches Institut der Universität Bonn
  • Brian E. S. Gunning
    • Plant Cell Biology Group, Research School of Biological SciencesAustralian National University
    • Cooperative Research Centre for Plant ScienceAustralian National University
    • Plant Cell Biology Group, Research School of Biological SciencesAustralian National University
    • Cooperative Research Centre for Plant ScienceAustralian National University
Article

DOI: 10.1007/BF01283289

Cite this article as:
Mews, M., Sek, F.J., Moore, R. et al. Protoplasma (1997) 200: 128. doi:10.1007/BF01283289

Summary

Cyclin proteins are components of the regulatory system that controls the orderly progression of the events of cell division. Their sub-cellular location, as well as their fluctuating abundance and their affinities for the cyclin-dependent kinases (CDKs) to which they bind, determine their successive roles during the cell cycle. Here we employ species-specific antibodies to monitor changes in quantity and location of four maize cyclins and maize Cdc2-kinase in dividing maize root tip cells. Maize cyclin Ia occurs in the nuclear matrix and is released when the nuclear envelope breaks down. In contrast, cyclin Ib is cytoplasmic until prophase; it associates transiently with the nuclear envelope and preprophase band (PPB) just before these structures break down and then associates with the condensed chromosomes and spindle region before declining at anaphase. Cyclin II and Cdc2 also occur in the PPB. Occurrence of cyclin Ib and Cdc2 at the PPB concurrent with initiation of breakdown is consistent with previous studies in which microinjection of cyclin-dependent protein kinase indicated that removal of the PPB at the time of nuclear-envelope breakdown is catalysed by a CDK. While cyclins Ia and III are predominantly nuclear prior to mitosis, cyclins Ib and II are predominantly cytoplasmic until prophase then become nuclear. The initial cytoplasmic retention of cyclins Ib and II correlates with their possession of a sequence similar to the cytoplasmic-retention signal of animal cyclin B1. Cyclin II binds to all microtubule arrays during the cell cycle, becoming markedly concentrated in the phragmoplast, and cyclin III associates with the spindle and then the phragmoplast. Cdc2 also occurs in the phragmoplast. Persistence of mitotic cyclins and CDK after mitosis into the cytokinetic stage, as seen in maize, is not paralleled in animal cells, where the cytokinetic mid-body is not so labelled, presumably reflecting the key role of the phragmoplast apparatus in plant cell division.

Keywords

Plant cyclinCyclin BPhragmoplastPreprophase bandCytoplasmic-retention signalSpindle

Abbreviations

CDK

cyclin-dependent kinase

CRS

cytoplasmicretention signal

NE

nuclear envelope

NEB

nuclear-envelope breakdown

NLS

nuclear-location signal

PPB

preprophase band

FITC

fluorescein isothiocyanate

TRITC

tetramethylrhodamine isothiocyanate

Copyright information

© Springer-Verlag 1997