Experimental studies on the function of the cortical cytoplasmic zone of the preprophase microtubule band
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Centrifugation of young seedlings ofTriticum durum andTriticum aestivum for 8–10 hours at 1,500–2,000 x g causes a serious disorder of the spatial organelle relationships in the interphase as well as the preprophase and mitotic subsidiary cell mother cells (SMCs). The nucleus, most organelles and cytoplasm are displaced to the centrifugal end of the cell, while the vacuoles lie at the other end. However, after centrifugation, the preprophase microtubule bands (PMBs) are nucleated and remain at the expected position close to the guard cell mother cells (GMCs). In some elongated SMCs the PMBs become completely separated from the nucleus. The mitotic spindle exhibits variable orientation and is usually formed at some distance from the PMB cortical zone.
Cytokinesis in SMCs is spatially highly disturbed and the cell plate shows a variety of unpredictable dispositions, which seem to be determined by: 1. the position of the preprophase-prophase nucleus and the orientation of the mitotic spindle as well as their spatial relationships to the PMB cortical zone, and 2. the space available for cell plate growth. Many of the daughter cells exhibit a highly variable shape and size in different planes. Usually one edge of the cell plate partly or totally joins the anticlinal parent wall adjacent to the PMB cortical zone.
In some SMCs ofZea mays andTriticum aestivum, the junction regions of the periclinal walls with the anticlinal ones, lined by the PMB cortical zone in normal SMCs, are detectably thickened after the arrest of mitosis and the prevention of interphase microtubule formation by a prolonged colchicine treatment. In a small number of protodermal cells of the same plants, participating in the development of stomatal complexes, irregular wall bodies or incomplete wall sheets were formed at wall regions lined by the PMB cortical zone.
The presented observations are in line with the following hypotheses: 1. the PMB cortical zone interacts with the growing edges of the cell plate “attracting” it to fuse with the underlying parent wall when the latter approaches the former at a critical distance, and 2. in SMCs particular regions of the PMB cortical zone and/or the adjacent plasmalemma promote the local wall deposition in the absence of microtubules.
- Andrews, F. M., 1915: Die Wirkung der Zentrifugalkraft auf Pflanzen. Jahrb. wiss. Bot.56, 221–253.
- Bailey, I. W., 1920: The cambium and its derivative tissues. III. A reconnaissance of cytological phenomena in the cambium. Amer. J. Bot.7, 417–434.
- Bouck, B. G., 1963: Stratification and subsequent behavior of plant cell organelles. J. Cell Biol.18, 441–457.
- Bünning, E., Biegert, F., 1953: Die Bildung der Spaltöffnungsinitialen beiAllium cepa. Z. für Botanik41, 17–39.
- Burgess, J., Northcote, D. H., 1968: The relationship between the endoplasmic reticulum and microtubular aggregation and disaggregation. Planta80, 1–14.
- Esau, K., Gill, R. H., 1965: Observations on cytokinesis. Planta67, 168–181.
- Galatis, B., 1977: Differentiation of stomatal meristemoids and guard cell mother cells into guard-like cells inVigna sinensis leaves after colchicine treatment. Planta136, 103–114.
- —, 1980: Microtubules and guard-cell morphogenesis inZea mays L. J. Cell Sci.45, 211–244.
- —, 1982: The organization of microtubules in guard cell mother cells ofZea mays L. Can. J. Bot.60, 1148–1166.
- —,Mitrakos, K., 1979: On the differential divisions and preprophase microtubule bands involved in the development of stomata ofVigna sinensis. J. Cell Sci.37, 11–37.
- — —, 1980: The ultrastructural cytology of the differentiating guard cells ofVigna sinensis. Amer. J. Bot.67, 1243–1261.
- —,Apostolakos, P., Katsaros, Chr., 1983 a: Synchronous organization of two preprophase microtubule bands and final cell plate arrangement in subsidiary cell mother cells of someTriticum species. Protoplasma117, 24–39.
- Galatis, B., Apostolakos, P., Katsaros, Chr., 1983b: Microtubules and their organizing centres in differentiating guard cells ofAdiantum capillus veneris. Protoplasma115, 176–192.
- - - - 1984: Positional inconsistency between preprophase microtubule band and final cell plate arrangement during subsidiary cell and hair cell formation in twoTriticum species. Can. J. Bot. (in press).
- — — —,Loukari, H., 1982: Preprophase microtubule band and local wall thickening in guard cell mother cells of someLeguminosae. Ann. Bot.50, 779–791.
- Gunning, B. E. S., 1981: Microtubules and cytomorphogenesis in a developing organ: The root primordium ofAzolla pinnata. In: Cytomorphogenesis in plants (Kiermayer, O., ed.), pp. 301–325. Wien-New York: Springer.
- —, 1983: The cytokinetic apparatus: Its development and spatial regulation. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 229–292. London-New York: Academic Press.
- —,Hardham, A. R., 1982: Microtubules. Ann. Rev. Plant Physiol.33, 651–698.
- — —,Hughes, J. E., 1978 a: Pre-prophase bands of microtubules in all categories of formative and proliferative cell divisions inAzolla roots. Planta143, 145–160.
- — — —, 1978 b: Evidence for initiation of microtubules in discrete regions of the cell cortex inAzolla root-tip cells, and an hypothesis on the development of cortical arrays of microtubules. Planta143, 161–179.
- Hardham, A. R., Gunning, B. E. S., 1980: Some effects of colchicine on microtubules and cell division in roots ofAzolla pinnata. Protoplasma102, 31–51.
- Hepler, P. K., 1981: Morphogenesis of tracheary elements and guard cells. In: Cytomorphogenesis in plants (Kiermayer, O., ed.), pp. 327–347. Wien-New York: Springer.
- —,Palevitz, B. A., 1974: Microtubules and microfilaments. Ann. Rev. Plant Physiol.25, 309–362.
- Miehe, H., 1899: Histologische und experimentelle Untersuchungen über die Auflage der Spaltöffnungen einiger Monokotylen. Bot. Centralbl.78, 321–331, 385–393.
- —, 1901: Ueber die Wanderungen des Pflanzlichen Zellkernes. Flora88, 105–142.
- Mottier, D. M., 1899: The effect of centrifugal force upon the cell. Ann. Bot.13, 325–361.
- Ootaki, T., 1963: Modification of the developmental axis by centrifugation inPteris vittata. Cytologia28, 21–29.
- Ôta, T., 1961: The role of cytoplasm in cytokinesis of plant cells. Cytologia26, 428–447.
- Palevitz, B. A., Hepler, P. K., 1974 a: The control of the plane of division during stomatal differentiation inAllium. I. Spindle reorientation. Chromosoma46, 297–326.
- — —, 1974 b: The control of the plane of division during stomatal differentiation inAllium. II. Drug studies. Chromosoma46, 327–341.
- Pickett-Heaps, J. D., 1969: Preprophase microtubules and stomatal differentiation. Some effects of centrifugation on symmetrical and asymmetrical cell division. J. Ultrastruct. Res.27, 24–44.
- —, 1974: Plant microtubules. In: Dynamic aspects of plant ultrastructure (Robards, A. W., ed.), pp. 219–251. London: McGraw-Hill.
- Schnepf, E., 1983: Morphogenesis in moss protonema. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 321–344. London-New York: Academic Press.
- Schmiedel, G., Schnepf, E., 1979: Side branch formation and orientation in the caulonema of the moss,Funaria hygrometrica: Experiments with inhibitors and with centrifugation. Protoplasma101, 47–59.
- Schmiedel, G., Schnepf, E., 1980: Polarity and growth of caulonema tip cells of the mossFunaria hygrometrica. Planta147, 405–413.
- Sinnott, E. W., Bloch, R., 1941: Division of vacuolate plant cells. Amer. J. Bot.28, 225–232.
- Experimental studies on the function of the cortical cytoplasmic zone of the preprophase microtubule band
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