Protoplasma

, Volume 206, Issue 1, pp 201–206

Visualization of microtubules in living cells of transgenicArabidopsis thaliana

Authors

  • K. Ueda
    • Graduate School of Biological ScienceNara Institute of Science and Technology
  • T. Matsuyama
    • Graduate School of Biological ScienceNara Institute of Science and Technology
  • T. Hashimoto
    • Graduate School of Biological ScienceNara Institute of Science and Technology
Rapid Communication

DOI: 10.1007/BF01279267

Cite this article as:
Ueda, K., Matsuyama, T. & Hashimoto, T. Protoplasma (1999) 206: 201. doi:10.1007/BF01279267

Summary

Microtubules (MTs) were visualized in living cells of several tissues in transgenicArabidopsis thaliana. The transformed Arabidopsis plant was obtained by infecting it withAgrobacterium tumefaciens carrying the GFP-TUA6 plasmid. The fluorescence of the MTs was due to the fluorescence of GFP-TUA6 that was polymerized into the MTs. The distribution patterns of the visualized MTs in the living epidermal cells of leaves was similar to that in fixed epidermal cells. The actual destruction of MTs by oryzalin was observed in a living cell. Cytochalasin B exerts no effect on the distribution pattern of MTs. The fluorescence intensity of MTs was different among cells in different tissues.

Keywords

CytoskeletonEpidermal cellGFP-TUA6 fusion proteinMicrotubuleTransgenic plantsTubulin

Copyright information

© Springer-Verlag 1999