Article

Protoplasma

, Volume 97, Issue 4, pp 337-349

First online:

Determination of the sodium, potassium and chloride ion concentrations in the chloroplasts of the halophyteSuaeda maritima by non-aqueous cell fractionation

  • Diana M. R. HarveyAffiliated withSchool of Biology, University of Sussex
  • , T. J. FlowersAffiliated withSchool of Biology, University of Sussex

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Summary

Leaf material from the halophyteSuaeda maritima L. Dum. grown under both saline and non-saline conditions was fractionated under non-aqueous conditions in order to determine the ion content of various subcellular compartments. Fractions containing cell walls, nuclei and chloroplasts were successfully prepared and contents of DNA, chlorophyll, protein and Na+, K+, and Cl determined. The cell wall fraction was not apparently heavily contaminated by the other fractions and had a low ion content although the nuclear fraction was contaminated by other organelles. The ion contents of chloroplasts were determined and the results discussed in relation to earlier microscopical data.