Protoplasma

, Volume 134, Issue 2, pp 111–120

Transport of rosettes from the golgi apparatus to the plasma membrane in isolated mesophyll cells ofZinnia elegans during differentiation to tracheary elements in suspension culture

  • C. H. Haigler
  • R. M. BrownJr.
Article

DOI: 10.1007/BF01275709

Cite this article as:
Haigler, C.H. & Brown, R.M. Protoplasma (1986) 134: 111. doi:10.1007/BF01275709

Summary

Rosettes of six particles have been visualized by freeze-fracture in the protoplasmic fracture (PF) faces of: a) the plasma membrane, b) Golgi cisternae, and c) Golgi-derived vesicles in mesophyll cells ofZinnia elegans that had been induced to differentiate synchronously into tracheary elements in suspension culture. These rosettes have been observed previously in the PF face of the plasma membranes of a variety of cellulose-synthesizing cells and are thought to be important in cellulose synthesis. InZinnia tracheary elements, the rosettes are localized in the membrane over regions of secondary wall thickening and are absent between thickenings. The observation of rosettes in the Golgi cisternae and vesicles suggests that the Golgi apparatus is responsible for the selective transport and exocytosis of rosettes in higher plants, as has been previously indicated in the algaMicrasterias (Giddingset al. 1980). The data presented indicate that the Golgi apparatus has a critical role in the control of cell wall deposition because it is involved not only in the synthesis and export of matrix components but also in the export of an important component of the cellulose synthesizing apparatus. The rosettes are present in the plasma membrane and Golgi vesicles throughout the enlargement of the secondary thickening, suggesting that new rosettes must be continually inserted into the membrane to achieve complete cell wall thickening.

Keywords

Cellulose synthesisGolgi apparatusRosettesTracheary elementsZinnia elegans

Abbreviations

EF

Golgi vesicles, exoplasmic fracture; the plasma membrane, extracellular fracture

PF

protoplasmic fracture

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • C. H. Haigler
    • 1
  • R. M. BrownJr.
    • 1
  1. 1.Department of BotanyThe University of Texas at AustinAustin
  2. 2.Department of Biological SciencesTexas Tech UniversityLubbockUSA