Journal of Neurocytology

, Volume 23, Issue 4, pp 218–233

Axotomy induces intranuclear immunolocalization of neuron-specific enolase in facial and hypoglossal neurons of the rat

Authors

  • D. N. Angelov
    • Institut I für AnatomieNasen- und Ohrenheilkunde der Universität zu Köln
  • W. F. Neiss
    • Institut I für AnatomieNasen- und Ohrenheilkunde der Universität zu Köln
  • A. Gunkel
    • Klinik für Hals-, Nasen- und Ohrenheilkunde der Universität zu Köln
  • O. Guntinas-Lichius
    • Institut I für AnatomieNasen- und Ohrenheilkunde der Universität zu Köln
  • E. Stennert
    • Klinik für Hals-, Nasen- und Ohrenheilkunde der Universität zu Köln
Article

DOI: 10.1007/BF01275526

Cite this article as:
Angelov, D.N., Neiss, W.F., Gunkel, A. et al. J Neurocytol (1994) 23: 218. doi:10.1007/BF01275526

Summary

Neuron-specific enolase as an enzyme of the glycolytic pathway is localized in the cytoplasm of nerve cells, but not in the cell nucleus. We have applied immunocytochemistry with 1∶64 000 polyclonal anti-rat neuron-specific enolase to the brainstem of male and female adult Wistar rats following: (a) transection of the facial nerve with immediate microsurgical nerve suture (facial-facial anastomosis), (b) transection of the hypoglossal nerve with immediate suture (hypoglossal-hypoglossal anastomosis) and (c) transection of the facial and hypoglossal nerve with immediate suture of the proximal hypoglossal to the distal facial nerve stump (hypoglossal-facial anastomosis). Studying the intracellular immunolocalization of neuron-specific enolase in neurons of the facial and hypoglossal nucleus we detected that (1) in normal rats about 20% of all facial and hypoglossal neurons display not only cytoplasmic, but also intranuclear neuron-specific enolase-like immunoreactivity and (2) following any axotomy of the facial or hypoglossal peripheral nerve, the perikarya of all injured motoneurons react by an outstanding increase of neuron-specific enolase-like immunoreactivity in the karyoplasm. Similar findings were obtained in experiments on non-fixed cultured Neuro-2a cells that had been lesioned with hydrogen peroxide. Counting the absolute numbers of normal and reactive neurons at 1–365 days post axotomy revealed that the increase of neuron-specific enolase in neuronal cell nuclei is temporary and reversible. It is first detected at 2 days post axotomy, reaches its maximum at 10–18 days post axotomy and is no longer evident 56 days following surgery. These findings suggest that the intranuclear neuron-specific enolase-like immunoreactive material may serve a regulatory function on the genome.

Download to read the full article text

Copyright information

© Chapman and Hall 1994