Planta

, Volume 201, Issue 1, pp 43–52

Factors regulating the expression of cell cycle genes in individual buds ofPopulus

  • Antje Rohde
  • Marc Van Montagu
  • Dirk Inzé
  • Wout Boerjan
Article

DOI: 10.1007/BF01258679

Cite this article as:
Rohde, A., Van Montagu, M., Inzé, D. et al. Planta (1997) 201: 43. doi:10.1007/BF01258679

Abstract

The early and differential responses of the individual buds along a shoot have remained largely unknown due to the difficulties of analyzing early indicators that allow the monitoring of the effects of subtle changes in the environment on the growth activity of the individual bud. To overcome this problem, we transformed poplar [Populus tremula (L.) xP. alba (L.)] with two chimeric genes,Pcdc2a-gus andPcycl At-gus, the expression of which is closely linked to cell division inArabidopsis thaliana (L.) Heynh. We analyzed the expression levels of both chimeric genes in individual buds of the same tree, and under different conditions known to promote or retard growth in the buds. The expression levels of both chimeric genes were found to reflect closely the growth activity of the buds. After decapitation of the shoot, the expression ofPcdc2a-gus andPcycl At-gus revealed rapid and selective changes in the cell cycle, even when no morphological changes were observed. Furthermore, on the basis of the expression of the chimeric cell cycle genes, different degrees of growth activity and dormancy could be discriminated in the axillary buds. In addition, the expression ofPcycl At-gus was found to be closely associated with the day length, which is critical for dormancy induction in poplar.

Key words

Bud dormancy Cell cycle Populus 

Abbreviations

GUS

β-glucuronidase

MU

methylumbelliferone

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Antje Rohde
    • 1
  • Marc Van Montagu
    • 1
  • Dirk Inzé
    • 2
  • Wout Boerjan
    • 1
  1. 1.Laboratorium voor Genetica, Department of GeneticsFlanders Interuniversity Institute for Biotechnology, Universiteit GentGentBelgium
  2. 2.Laboratoire Associé de l'Institut National de la Recherche Agronomique (France)Universiteit GentGentBelgium

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