Article

Molecular Breeding

, Volume 1, Issue 4, pp 397-410

First online:

The inheritance and chromosomal localization of AFLP markers in a non-inbred potato offspring

  • Herman J. van EckAffiliated withDepartment of Plant BreedingThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University Email author 
  • , Jeroen Rouppe van der VoortAffiliated withDepartment of NematologyThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
  • , Jan DraaistraAffiliated withDepartment of NematologyThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
  • , Peter van ZandvoortAffiliated withDepartment of NematologyThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
  • , Ellen van EnckevortAffiliated withDepartment of NematologyThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
  • , Bart SegersAffiliated withKeygene N.V.The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
  • , Johan PelemanAffiliated withKeygene N.V.The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
  • , Evert JacobsenAffiliated withDepartment of Plant BreedingThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
  • , Johannes HelderAffiliated withDepartment of NematologyThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University
    • , Jaap BakkerAffiliated withDepartment of NematologyThe Graduate School of Experimental Plant Sciences, Wageningen Agricultural University

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Abstract

AFLPTM is a new technique to generate large numbers of molecular markers for genetic mapping. The method involves the selective amplification of a limited number of DNA restriction fragments out of complex plant genomic DNA digests using PCR. With six primer combinations 264 segregating AFLP amplification products were identified in a diploid backcross population from non-inbred potato parents. The identity of an AFLP marker was specified by the primer combination of the amplification product and its size estimated in bases. The segregating AFLP amplification products were mapped by using a mapping population with 217 already known RFLP, isozyme and morphological trait loci. In general, the AFLP markers were randomly distributed over the genome, although a few clusters were observed. No indications were found that AFLP markers are present in other parts of the genome than those already covered by RFLP markers. Locus specificity of AFLP markers was demonstrated because equally sized amplification products segregating from both parental clones generally mapped to indistinguishable maternal and paternal map positions. Locus specificity of AFLP amplification products will allow to establish the chromosomal identity of linkage groups in future mapping studies.

Since AFLP technology is a multi-locus detection system, it was not possible to identify the AFLP alleles which belong to a single AFLP locus. The consequences of a genetic analysis based on single alleles, rather than on loci with two or more alleles on mapping studies using progenies of non-inbred parents are discussed.

Key words

AFLP marker allelism allogamous linkage map Solanum tuberosum