Journal of Neural Transmission

, Volume 73, Issue 1, pp 7–21

Human dopamine receptor subtypes—in vitro binding analysis using3H-SCH 23390 and3H-raclopride

  • H. Hall
  • L. Farde
  • G. Sedvall
Original Papers

DOI: 10.1007/BF01244618

Cite this article as:
Hall, H., Farde, L. & Sedvall, G. J. Neural Transmission (1988) 73: 7. doi:10.1007/BF01244618

Summary

Affinities and regional densities of the D1- and D2-dopamine receptor subtypes were studied in the human post-mortem brain in vitro using the two selective radioligands3H-SCH23390 and3H-raclopride.3H-Raclopride binding was confined to the caudate nucleus, the putamen and the substantia nigra, while3H-SCH23390 bound to cortical regions as well. The binding of3H-SCH 23390 was reduced by a low concentration of ketanserin, indicating binding to 5-HT2 receptors in addition to the D1dopamine receptors. The endogenous neurotransmitter dopamine interacted potently both with the D1-dopamine receptor and the D2-dopamine receptor, displaying two affinity states for each subtype. The distribution of the dopamine receptor subtypes obtained in the present in vitro investigation is in agreement with data obtained with11C-SCH 23390 and11C-raclopride in positron emission tomographic studies in human volunteers.

Keywords

Human brain post-mortem dopamine receptors 3H-SCH23390 receptor binding 3H-raclopride receptor binding 

Copyright information

© Springer Verlag 1988

Authors and Affiliations

  • H. Hall
    • 1
  • L. Farde
    • 2
  • G. Sedvall
    • 2
  1. 1.Department of Biochemical NeuropharmacologyCNS-Drugs, Research and Development LaboratoriesSödertÄlje
  2. 2.Department of Psychiatry and PsychologyKarolinska InstituteStockholmSweden