, Volume 32, Issue 1, pp 73-81

Plaque assay of variola virus in a cynomolgus monkey kidney cell line

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Variola virus produced plaques in a cynomolgus monkey kidney cell line, JINET, under agar overlay medium. The plaque morphology of variola virus in these cells was clearly distinct from that of other members of the variola-vaccinia subgroup of poxvirus and the plaque morphology marker of variola virus may be used for the identification of variola virus. The plaque number of variola virus was enhanced by the addition of DEAE-dextran and MgCl2 to agar overlay medium at concentrations of 100 to 200 Μg/ml and 15 to 45 mm, respectively. The sensitivity of the plaque method in the presence of 100 Μg/ml of DEAE-dextran and 25 mm MgCl2 in the overlay medium was equal to that of the usual CAM method.