Brief Communication

Somatic Cell and Molecular Genetics

, Volume 16, Issue 5, pp 501-505

Localization of gene for human syndecan, an integral membrane proteoglycan and a matrix receptor, to chromosome 2

  • M. Ala-KapeeAffiliated withDepartment of Genetics, University of Helsinki
  • , H. NevanlinnaAffiliated withDepartment of Obstetrics and Gynecology, University of Helsinki
  • , M. MaliAffiliated withDepartment of Medical Biochemistry, University of Turku
  • , M. JalkanenAffiliated withDepartment of Medical Biochemistry, University of Turku
  • , J. SchröderAffiliated withDepartment of Genetics, University of Helsinki

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Abstract

Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. For chromosomal localization of the human syndecan gene, a panel of mouse-human somatic cell hybrids was analyzed by Southern blotting using the cDNA probe for human syndecan. The hybrids were karyotyped at the time of DNA extraction. A band corresponding to the human syndecan gene in Southern blots was found only in a hybrid cell line containing human chromosome 2. This hybrid was subcloned and its subclones were analyzed by Southern blotting and karyotyped. Subclones carrying human chromosome 2 contained the syndecan gene, while subclones not carrying this chromosome did not. The human syndecan gene is thus assigned to chromosome 2.