, Volume 9, Issue 2, pp 115-119

Ultrastructural morphometry of the pancreatic β-cell

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Standard stereological methods have been used to determine the morphometric parameters of organelles contained in theβ- cella of mouse pancreas. Sectioned material from ten islets was analysed at three levels of magnification in the electron microscope. Quantitative data was obtained about the volume number and surface area of the various cytological components and was expressed as density per cm3 of tissue and as absolute dimensions for the averageβ-cell. The organelles studied were the nucleus, cytoplasmic ground substance, rough and smooth endoplasmic reticulum, the ribosomes, mitochondria,β-granules and microtubules.