Journal of Assisted Reproduction and Genetics

, Volume 10, Issue 1, pp 82–90

A fast and efficient method for simultaneous X and Y in situ hybridization of human blastomeres

  • Santiago Munné
  • Heinz-Ulrich G. Weier
  • Jonathan Stein
  • Jamie Grifo
  • Jacques Cohen
Reproductive Genetics

DOI: 10.1007/BF01204446

Cite this article as:
Munné, S., Weier, HU.G., Stein, J. et al. J Assist Reprod Genet (1993) 10: 82. doi:10.1007/BF01204446

Purpose

To use a 6-hr fluorescence in situ hybridization (FISH) procedure involving fluorochrome-labeled probes to determine the gender of blastomeres from arrested biopsied human embryos.

Results

Simultaneous detection of X and Y chromosomes was performed on 68 blastomeres with this technique. The FISH efficiency for gender determination was 95.5% (65/68). In addition, rehybridization with chromosome 8-specific probes was performed to determine the ploidy of blastomeres with more than two sex chromosomes.

Conclusion

This technique offers an alternative to polymerase chain reaction for the preimplantation diagnosis of X-linked diseases and can also be used for ploidy assessment.

Key words

embryo preimplantation diagnosis X chromosome Y chromosome chromosome 8 

Copyright information

© Plenum Publishing Corporation 1993

Authors and Affiliations

  • Santiago Munné
    • 1
  • Heinz-Ulrich G. Weier
    • 3
  • Jonathan Stein
    • 1
  • Jamie Grifo
    • 1
  • Jacques Cohen
    • 1
  1. 1.The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and GynecologyNew York Hospital-Cornell University Medical CenterNew YorkUSA
  2. 2.The Gamete and Embryo Research LaboratoryCornell University Medical CollegeNew YorkUSA
  3. 3.Division of Molecular Cytometry MCB 230, Department of Laboratory MedicineUniversity of CaliforniaSan FranciscoUSA