Microchimica Acta

, Volume 88, Issue 1, pp 65–72

Electron microscopy of submicron particles in natural waters — Specimen preparation by centrifugation

  • Tsumota Nomizu
  • Atsushi Mizuike

DOI: 10.1007/BF01196688

Cite this article as:
Nomizu, T. & Mizuike, A. Mikrochim Acta (1986) 88: 65. doi:10.1007/BF01196688


Particles ranging from ca. 0.1 to 1.0μm in 5 ml of pond water are collected on a 10–20 nm carbon film on a gold or copper specimen grid, placed on a special holder in a 7-ml centrifuge tube, by centrifugation at 10000 r. p. m. for 18 min. The specimens thus prepared are reproducible and preserve the morphology of the particles satisfactorily. The proposed technique is more suitable than filtration, direct drying and spray drying techniques for morphological studies and elemental analysis of individual particles with scanning, transmission and analytical electron microscopes.

Key words

submicron particleselectron microscopycentrifugationnatural waters

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Tsumota Nomizu
    • 1
  • Atsushi Mizuike
    • 1
  1. 1.Faculty of EngineeringNagoya UniversityNagoyaJapan