Prolonged survival of transected nerve fibres in C57BL/Ola mice is an intrinsic characteristic of the axon
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Transected axons in C57BL/Ola mice survive for extraordinary lengths of time as compared to those of normal rodents. The biological difference in the substrain that confers the phenotype of prolonged axonal survival is unknown. Previous studies suggest the ‘defect’ to be a property of the nervous system itself, rather than one of haematogenous cells. Neuronal or non-neuronal elements could be responsible for this phenotype. This study was undertaken to determine whether Schwann cells, the most numerous of the non-neuronal cells intrinsic to the peripheral nerve, are responsible for delayed degeneration of transected axons. We created sciatic nerve chimeras by transplanting nerve segments between standard C57BL/6 and C57BL/Ola mice, allowing regeneration of host axons through the grafts containing donor Schwann cells. These nerves were then transected and the time course of axonal degeneration was observed. The results show that fast or slow degeneration is a property conferred by the host, and therefore cannot be ascribed to the Schwann cells. Similarly, transected C57BL/Ola axons in explanted dorsal root ganglia cultures survived longer than transected axons from standard mice. Taken together these results indicate that the responsible abnormality is intrinsic to the C57BL/Ola axon.
- Adams, D. O. &Hamilton, T. A. (1984) The cell biology of macrophage activation.Annual Review of Immunology 2, 283–318.
- Aguayo, A. J., Charron, L. &Bray, G. M. (1976a) Potential of Schwann cells from unmyelinated nerves to produce myelin: a quantitative ultrastructural and autoradiographic study.Journal of Neurocytology 5, 565–73.
- Aguayo, A. J., Epps, J., Charron, L. &Bray, G. M. (1976b) Multipotentiality of Schwann cells in cross anastomosed and grafted myelinated and unmyelinated nerves: quantitative microscopy and radioautography.Brain Research 104, 1–20.
- Aguayo, A. J., Bray, G. M. &Perkins, C. S. (1979) Axon-Schwann cell relationships in neuropathies of mutant mice.Annals of the New York Academy of Science 317, 512–31.
- Bisby, M. A. &Chen, S. (1990) Delayed Wallerian degeneration in sciatic nerves of C57BL/Ola mice is associated with impaired regeneration of sensory axons.Brain Research 530, 117–20.
- Bottenstein, J. E. &Sato, G. H. (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented medium.Proceedings of the National Academy of Sciences (USA) 76, 514–17.
- Clemence, A., Mirsky, R. &Jessen, K. R. (1989) Nonmyelin-forming Schwann cells proliferate rapidly during Wallerian degeneration in the rat sciatic nerve.Journal of Neurocytology 18, 185–92.
- Dewaegh, S. M., Lee, V. M.-Y. &Brady, S. T. (1992) Local modulation of neurofilament phosphorylation, axonal caliber, and slow axonal transport by myelinating Schwann cells.Cell 68, 451–63.
- Gainer, H., Tasaki, I. &Lasek, R. J. (1977) Evidence for the glia-neuron protein transfer hypothesis from intracellular perfusion studies of squid giant axons.Journal of Cell Biology 74, 524–30.
- Glass, J. D. &Griffin, J. W. (1991) Neurofilament redistribution in transected nerves: Evidence for bidirectional transport of neurofilaments.Journal of Neuroscience 11, 3146–54.
- Gutmann, E. &Holubar, J. (1950) The degeneration of peripheral nerve fibres.Journal of Neurology, Neurosurgery, and Psychiatry 13, 89–105.
- Joseph, B. (1973) Somatofugal events in Wallerian degeneration: a conceptual overview.Brain Research 59, 1–18.
- Lopachin, R. M., Lopachin, V. R. &Saubermann, A. J. (1990) Effects of axotomy on distribution and concentration of elements in rat sciatic nerve.Journal of Neurochemistry 54, 320.
- Lubinska, L. (1977) Early course of Wallerian degeneration in myelinated fibers of the rat phrenic nerve.Brain Research 130, 47–63.
- Lunn, E. R., Perry, V. H., Brown, M. C., Rosen, H. &Gordon, S. (1989) Absence of Wallerian degeneration does not hinder regeneration in peripheral nerve.European Journal of Neuroscience 1, 27–33.
- Oaklander, A. L., Miller, M. S. &Spencer, P. S. (1987) Early biochemical changes in degenerating móuse sciatic nerve are associated with endothelial cells.Brain Research 419, 39–45.
- Pellegrino, R. G. &Spencer, P. S. (1985) Schwann cell mitosis in response to regenerating peripheral axonsin vivo.Brain Research 341, 16–25.
- Perry, V. H., Brown, M. C., Lunn, E. R., Tree, P. &Gordon, S. (1990) Evidence that very slow Wallerian degeneration in C57BL/Ola mice is an intrinsic property of the peripheral nerve.European Journal of Neuroscience 2, 802–8.
- Perry, V. H., Brown, M. C. &Lunn, E. R. (1991) Very slow retrograde and Wallerian degeneration in the CNS of C57BL/Ola mice.European Journal of Neuroscience 3, 102–5.
- Ramon Y Cajal, S. (1928)Degeneration and regeneration of the nervous system (translated byR. M. May). London: Oxford University Press.
- Schlaepfer, W. W. &Bunge, R. P. (1973) The effects of calcium ion concentration on the degradation of amputated axons in tissue culture.Journal of Cell Biology 59, 456–70.
- Schlaepfer, W. W. &Zimmerman, U. P. (1984) Calcium-activated protease and the regulation of the axonal cytoskeleton. InAxonal transport in neuronal growth and regeneration (edited byElam, J. S. &Cancalon, P.) pp. 261–73. New York: Plenum Press.
- Seitz, R. J., Reiners, K., Himmelmann, F., Heininger, K., Hartung, H.-P. &Toyka, K. V. (1989) The blood-nerve barrier in Wallerian degeneration: a sequential long-term study.Muscle and Nerve 12, 627–35.
- Singer, M. &Steinberg, M. C. (1972) Wallerian degeneration: a reevaluation based on transected and colchicinepoisoned nerves in the amphibian,Triturus.American Journal of Anatomy 133, 51–84.
- Stoll, G., Griffin, J. W., Li, C. Y. &Trapp, B. D. (1989) Wallerian degeneration in the peripheral nervous system: participation of both Schwann cells and macrophages in myelin degradation.Journal of Neurocytology 18, 671–83.
- Trapp, B. D., Hauer, P. &Lemke, G. (1988) Axonal regulation of myelin protein mRNA levels in actively myelinating Schwann cells.Journal of Neuroscience 8, 3515–21.
- White, F. V., Toews, A. D., Goodrum, J. F., Novicki, D. L., Bouldin, T. W. &Morell, P. (1989) Lipid metabolism during early stages of Wallerian degeneration in the rat sciatic nerve.Journal of Neurochemistry 52, 1085–92.
- Williams, P. L. &Hall, S. M. (1971) Chronic Wallerian degeneration — Anin vivo and ultra structural study.Journal of Anatomy 109, 487–503.
- Windebank, A. J., Word, P., Bunge, R. P. &Dyck, P. J. (1985) Myelination determines the caliber of dorsal root ganglion neurons in culture.Journal of Neuroscience 6, 1563–7.
- Prolonged survival of transected nerve fibres in C57BL/Ola mice is an intrinsic characteristic of the axon
Journal of Neurocytology
Volume 22, Issue 5 , pp 311-321
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- 1. Department of Neurology, The Johns Hopkins University School of Medicine, 21287-6965, Baltimore, MD
- 2. Department of Pathology, The Johns Hopkins University School of Medicine, 21287-6965, Baltimore, MD
- 3. Department of Orthopedic Surgery, The Johns Hopkins University School of Medicine, 21287-6965, Baltimore, MD
- 4. Department of Neuroscience, The Johns Hopkins University School of Medicine, 21287-6965, Baltimore, MD