Evidence for supporting cell mitosis in response to acoustic trauma in the avian inner ear
- Y. Raphael
- … show all 1 hide
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
Acoustic overstimulation can lead to sensory cell (hair cell) loss in the auditory epithelium. Damaged hair cells in the organ of Corti (the mammalian auditory end-organ) degenerate and are replaced by non-sensory cells (supporting cells) which construct an irreversible scar. In birds, however, auditory hair cells which are damaged by acoustic trauma or ototoxic drugs may be replaced by new hair cells. As first step in determining the mechanism of hair cell regeneration, we developed an assay for cell divisions in the auditory epithelium after acoustic trauma. The results of these experiments demonstrate that supporting cells in damaged regions of the auditory epithelium incorporate the DNA-specific marker bromodeoxyuridine as early as one day after noise exposure. We provide direct evidence that following acoustic insult to the avian inner ear, supporting cells which reside within the sensory epithelium divide near the luminal surface and repopulate the epithelium. These results suggest that supporting cells participate in scar formation during hair cell degeneration, and produce new cells for regeneration.
- Balak, K. J., Corwin, J. T. &Jones, J. E. (1990) Regenerated hair cells can originate from supporting cell progeny: evidence from phototoxicity and laser ablation experiments in the lateral line system.Journal of Neuroscience 10, 2502–12.
- Bohne, B. A. (1976) Healing of the noise damaged inner ear. InHearing and Davis: Essays Honoring Hallowel Davis (edited byHirsh, S. K., Eldredge, D. H., Hirsh, I. J. &Silverman, S. R.) pp. 85–96. Saint Louis: Washington University Press.
- Corwin, J. T. (1981) Postembryonic production and aging of inner ear hair cells in sharks.Journal of Comparative Neurology 201, 541–53.
- Corwin, J. T. &Cotanche, D. A. (1988) Regeneration of sensory hair cells after acoustic trauma.Science 240, 1772–4.
- Corwin, J. T. &Warchol, M. E. (1991) Auditory hair cells: structure, function, development, and regeneration.Annual Reviews in Neuroscience 14, 301–33.
- Cotanche, D. A. &Dopyera, C. E. J. (1990) Hair cell and supporting cells response to acoustic trauma in the chick cochlea.Hearing Research 46, 29–40.
- Craig-Holmes, A. P. &Shaw, M. W. (1976) Cell cycle analysis in asynchronous cultures using the BrdU-Hoechst technique.Experimental Cell Research 99, 79–87.
- Forge, A. (1985) Outer hair cell loss and supporting cell expansion following chronic gentamicin treatment.Hearing Research 19, 171–82.
- Ginzberg, D. R. &Giluea, B. N. (1979) Modulation of cell junctions during differentiation of the chicken otocyst sensory epithelium.Developmental Biology 68, 110–29.
- Girod, D. A., Duckert, L. G. &Rubel, E. W. (1989) Possible precursors of regenerated hair cells in the avian cochlea following acoustic trauma.Hearing Research 42, 175–94.
- Gratzner, H. G. (1982) Monoclonal antibody to 5-bromo and 5-iododeoxy-uridine: a new reagent for detection of DMA replication.Science 218, 474–5.
- Hawkins, J. E. Jr &Johnson, L.-G. (1981) Histopathology of cochlear and vestibular ototoxicity in laboratory animals. InAminoglycoside Ototoxicity (edited byLerner, S. A., Matz, J. G. &Hawkins, J. E., Jr) pp. 175–95. Boston: Little, Brown and Company.
- Marsh, R. R., Xu, L., Moy, J. P. &Saunders, J. C. (1990) Recovery of the basilar papilla following intense sound exposure in the chick.Hearing Research 46, 229–38.
- Raphael, Y. (1991) Damage to the tectorial membrane may protect chick hair cells from noise overstimulation.Hearing Research 53, 173–84.
- Raphael, Y. &Altschuler, R. A. (1991) Modulation of cytoskeletal and junctional proteins during hair cell degeneration.Cell Motility and the Cytoskeleton 18, 215–27.
- Raphael, Y. &Altschuler, R. A. (1992) Early microfilament reorganization in injured auditory epithelia.Experimental Neurology 115, 32–6.
- Rouse, G. W. &Pickles, J. O. (1991) Paired development of hair cell in the neuromasts of the teleost lateral line.Proceedings of the Royal Society of London (Series B) 246, 123–8.
- Ruben, R. J. (1967) Development of the inner ear of the mouse: a radioautographic study of terminal mitoses.Acta Oto-laryngologica (Suppl)220, 1–44.
- Ryals, B. M. &Rubel, E. W. (1988) Hair cell regeneration after acoustic trauma in adultCoturnix quail.Science 240, 1774–6.
- Sauer, F. C. (1935) Mitosis in the neural tube.Journal of Comparative Neurology 62, 377–405.
- Saunders, J. C., Dear, S. P. &Schneider, M. E. (1985) The anatomical consequences of acoustic injury: a review and tutorial.Journal of the Acoustical Society of America 78, 833–60.
- Saunders, J. C., Cohen, Y. E. &Szymko, Y. M. (1991) The structural and functional consequences of acoustic injury in the cochlea and peripheral auditory system: a five year update.Journal of the Acoustical Society of America 90, 136–46.
- Evidence for supporting cell mitosis in response to acoustic trauma in the avian inner ear
Journal of Neurocytology
Volume 21, Issue 9 , pp 663-671
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Y. Raphael (1)
- Author Affiliations
- 1. Kresge Hearing Research Institute, The University of Michigan, 1301 East Ann Street, 48109, Ann Arbor, Michigan, USA