Biology of Metals

, Volume 3, Issue 3, pp 237–241

Novel effect of aromatic compounds on the iron-dependent expression of theEscherichia coli K12 manganese superoxide dismutase (sodA) gene

Authors

  • Eric C. Niederhoffer
    • Stable Isotope ResourceIsotope and Structural Chemistry Group (INC-4)
  • James A. Fee
    • Stable Isotope ResourceIsotope and Structural Chemistry Group (INC-4)
Original articles

DOI: 10.1007/BF01140586

Cite this article as:
Niederhoffer, E.C. & Fee, J.A. Biol Metals (1990) 3: 237. doi:10.1007/BF01140586

Summary

InEscherichia coli, the superoxide dismutase genes (sodA andsodB) sense the availability of Fe through the action of thefur locus [E. C. Niederhoffer, C. M. Naranjo, K. L. Bradley, J. A. Fee (1990) Control ofEscherichia coli superoxide dismutases (sodA andsodB) genes by the ferric uptake regulation (fur) locus,J. Bacteriol. 172, 1930–1938]. Previous work from other laboratories has shown that a variety of metal chelators and of redox-active aromatic compounds can dramatically induce expression ofsodA. Here we show that non-redox-active, non-metal-chelating aromatic compounds also enhance expression of a chromosomalsodA gene fusion and that these effects are strongly modulated by the Fur phenotype (Fur±) and by the availability of iron in the culture medium. The compounds studied were ethidium bromide, hemin, 2,2′-bipyridine, 1,10-phenantroline, 4,7-phenantroline, rhodamine B1, rhodamine 6G, and, for comparison to previous studies, Paraquat.

Key words

Superoxide dismutasesAromatic compoundsFerric uptake regulation

Abbreviations

DTPA

diethylenetriaminepentaacetic acid

Paraquat

N,N′-dimethyl-1,1-bipyridene

bpy

2,2′-bipyridine

phen

1,10-phenanthroline

4,7-phen

4,7-phenanthroline

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Copyright information

© Springer-Verlag 1990