Original Articles

Biology of Metals

, Volume 1, Issue 1, pp 9-17

First online:

High-performance liquid chromatography of siderophores from fungi

  • Silvia Konetschny-RappAffiliated withMikrobiologie 1, Institut für Organische Chemie
  • , Hans-Georg HuschkaAffiliated withMikrobiologie 1, Institut für Biologie I
  • , Günther WinkelmanneAffiliated withMikrobiologie 1, Institut für Biologie I
  • , Günther JungAffiliated withMikrobiologie 1, Institut für Organische Chemie

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


A reversed-phase HPLC separation of iron(III) chelates of 16 representative fungal siderophores including ferrichromes, coprogens and triacetylfusarinine C was established in order to investigate siderophore production of fungi. For comparison purposes, the widely used bacterial siderophore ferrioxamine B was included. Culture filtrates of the fungiPenicillium resticulosum, Fusarium dimerum, Aspergillus fumigatus andNeurospora crassa were quantitatively analyzed for the presence of known and unknown siderophores after growth in low-iron culture media and adsorption on XAD-2 columns using this HPLC separation system. Photodiode array detection allowed the distinction between siderophores and non-siderophores. According to their ultraviolet/visible spectra, a further classification of the siderophores into four types due to the number of anhydromevalonic acid residues per molecule (0–3) was possible.

Key words

Siderophores HPLC separation Identification Fungi