Virchows Archiv A

, Volume 382, Issue 1, pp 21–30

Orcein positive granules in the hepatocytes in chronic intrahepatic cholestasis

Morphological, histochemical and electron X-ray microanalytical examination
  • Yasuni Nakanuma
  • Tetsuji Karino
  • Goroku Ohta
Original Articles

DOI: 10.1007/BF01102738

Cite this article as:
Nakanuma, Y., Karino, T. & Ohta, G. Virchows Arch. A Path. Anat. and Histol. (1979) 382: 21. doi:10.1007/BF01102738

Summary

The morphological characteristics of orcein positive granules in hepatocytes from 11 patients with chronic intrahepatic cholestasis and from 2 newborn normal livers were studied. Histochemical investigations revealed their protein nature and many sulphydryl and/or disulphide groups. Copper was demonstrated in the granules by histochemical techniques and electron X-ray micronalysis. No difference was observed in the hepatic distribution and appearance of the granules between the livers of those with chronic cholestasis and the newborn. Ultrastructurally, a variety of electron dense granules were seen at the site of orcein positive granules in the hepatocytes of the patient with primary biliary cirrhosis. Some had a single-layered membrane and seem to be lysosomal derivatives. It is suggested that the copper in lysosomes seen in both chronic cholestasis and normal newborn livers, need not to be cytotoxic.

Key words

Chronic intrahepatic cholestasisPrimary biliary cirrhosisHepatocellular orcein positive granulesCopper hepatocytotoxicity

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Yasuni Nakanuma
    • 1
  • Tetsuji Karino
    • 1
  • Goroku Ohta
    • 1
  1. 1.Second Department of Pathology, School of MedicineKanazawa UniversityKanazawaJapan