Enzymatic reactions in aqueous-organic media III. Peptide synthesis by α-chymotrypsin immobilized with polyvinyl alcohol in ethanol
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- Noritomi, H. & Kise, H. Biotechnol Lett (1987) 9: 383. doi:10.1007/BF01089001
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Immobilized α-chymotrypsin, noncovalently bound to polyvinyl alcohol (PVA), has been employed for synthesis of N-acetyl-L-tyrosinyl-L-glycine amide from N-acetyl-L-tyrosine and L-glycine amide in the homogenous system (water-ethanol), parallel to the use of the native enzyme. Native and immobilized enzymes showed the maximal yields of Ac-Tyr-Gly-NH2 of 35 % and 50 % respectively for a particular water concentration in a 24 h reaction at 30 °C.