Biotechnology Letters

, Volume 9, Issue 6, pp 383–386

Enzymatic reactions in aqueous-organic media III. Peptide synthesis by α-chymotrypsin immobilized with polyvinyl alcohol in ethanol

  • H. Noritomi
  • H. Kise
Article

DOI: 10.1007/BF01089001

Cite this article as:
Noritomi, H. & Kise, H. Biotechnol Lett (1987) 9: 383. doi:10.1007/BF01089001

Summary

Immobilized α-chymotrypsin, noncovalently bound to polyvinyl alcohol (PVA), has been employed for synthesis of N-acetyl-L-tyrosinyl-L-glycine amide from N-acetyl-L-tyrosine and L-glycine amide in the homogenous system (water-ethanol), parallel to the use of the native enzyme. Native and immobilized enzymes showed the maximal yields of Ac-Tyr-Gly-NH2 of 35 % and 50 % respectively for a particular water concentration in a 24 h reaction at 30 °C.

Copyright information

© Science and Technology Letters 1987

Authors and Affiliations

  • H. Noritomi
    • 1
  • H. Kise
    • 1
  1. 1.Institute of Materials ScienceUniversity of TsukubaNiihari, IbarakiJapan

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