Biotechnology Letters

, Volume 12, Issue 9, pp 673–678

Production of an extracellular chitinolytic system byTalaromyces emersonii CBS 814.70.

  • L. Hendy
  • J. Gallagher
  • A. Winters
  • T. J. Hackett
  • L. McHale
  • A. P. McHale
Article

DOI: 10.1007/BF01088192

Cite this article as:
Hendy, L., Gallagher, J., Winters, A. et al. Biotechnol Lett (1990) 12: 673. doi:10.1007/BF01088192

Summary

The thermophilic fungusTalaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced.

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • L. Hendy
    • 1
  • J. Gallagher
    • 1
  • A. Winters
    • 1
  • T. J. Hackett
    • 1
  • L. McHale
    • 1
  • A. P. McHale
    • 1
  1. 1.Dept. of Microbiology, Moyne InstituteTrinity CollegeDublin 2Ireland