Anti-hypertensive substances in fermented soybean, natto
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Natto is a traditional Japenese fermented food made by fermenting boiled soy beans withBacillus natto. Its contents of inhibitors against the angiotensin converting enzyme (ACE, EC126.96.36.199) were investigated. Relatively strong inhibitory activity (IC50:0.4 mg/ml, 11.8 inhibition units/g natto) was detected in natto extracts and the inhibitory activity observed in the viscous fraction was more potent than in the bean extract. Two groups of inhibitors in the viscous material, high and low molecular weight inhibitors, were resolved by dialysis test. The inhibitor of high molecular weight was a protein with low IC50 value (0.12 mg/ml). The two types of low molecular weight inhibitors were detected in ethanol extracts (IC50: 0.53 mg/ml and 0.95 mg/ml) and they were found to be stable over a wide range of pH and temperature up to 100°C. They were different in the mode of ACE inhibition. One is competitive, and the other noncompetitive against the hydrolysis of Bz-Gly-His-Leu by ACE.
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- Anti-hypertensive substances in fermented soybean, natto
Plant Foods for Human Nutrition
Volume 47, Issue 1 , pp 39-47
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers
- Additional Links
- Angiotensin converting enzyme (ACE)
- Fermented foods
- Reninangiotensin system
- Industry Sectors
- Author Affiliations
- 1. Department of Brewing and Fermentation, Tokyo University of Agriculture, Sakuragaoka, Setagaya, 156, Tokyo, Japan
- 2. Ministry of Agriculture, Forestry and Fishery, National Food Research Institute, Kannondai, 305, Tsukuba, Ibaraki, Japan