Plant Systematics and Evolution

, Volume 216, Issue 3, pp 325-331

First online:

Analysis of nucleolar organizer constitution by fluorescent in situ hybridization (FISH) in diploid and artificially produced haploid sporophytes of the fernOsmunda japonica (Osmundaceae)

  • Suzue M. KawakamiAffiliated withSeirei High School, Seireicho, Seto, Aichi Email author 
  • , Katsuhiko KondoAffiliated withLaboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University
  • , Shogo KawakamiAffiliated withFaculty of Education, Aichi University of Education

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HaploidOsmunda japonica (2n = × = 22), produced in tissue culture by induced apogamy and acclimatized in vivo, was cytologically compared with the diploid sister plant. The haploid had smaller guard cells than the diploid and a numerically exactly reduced karyotype. Fluorescent in situ hybridization (FISH) using an rDNA probe showed four hybridization signals (one large and three small ones) in the haploid and eight signals (two large and six small ones) in the diploid plant. These results represent a cytological proof for the origin of the haploid plant by apogamy without recognizable chromosome aberrations.

Key words

Pteridophyta Osmundaceae Osmunda japonica Ferns induced apogamy haploid FISH