Article

Plant Systematics and Evolution

, Volume 216, Issue 3, pp 243-249

First online:

DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi

  • Oscar F. CuberoAffiliated withDepartamento de Biologia Vegetal II, Universidad Complutense
  • , Ana CrespoAffiliated withDepartamento de Biologia Vegetal II, Universidad Complutense
  • , Jamshid FatehiAffiliated withCABI Bioscience UK Centre (Egham)
  • , Paul D. BridgeAffiliated withCABI Bioscience UK Centre (Egham)

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Abstract

This paper presents a DNA extraction method suitable for fresh, herbarium-stored, lichenized and other fungal specimens. The method is fast and reliable, comparatively inexpensive and is suitable for obtaining PCR amplification quality DNA from large numbers of samples in a short time. The method has been tested with over 300 samples ofAscochyta, Phyllosticta, Ramalina, Parmelia andPhysconia. Amplifiable fungal DNA was extracted from pure cultures, leaf lesions, whole thalli and dissected “only-fungal” sections of lichenized fungi. In addition, the method has proved suitable for use with herbarium specimens of both lichenized and non-lichenized fungi, stored as dried pure cultures or dried infected plant material.

Key words

Herbarium DNA extraction filamentous fungi lichenized fungi