Plant Systematics and Evolution

, Volume 216, Issue 3, pp 243–249

DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi

  • Oscar F. Cubero
  • Ana Crespo
  • Jamshid Fatehi
  • Paul D. Bridge
Article

DOI: 10.1007/BF01084401

Cite this article as:
Cubero, O.F., Crespo, A., Fatehi, J. et al. Pl Syst Evol (1999) 216: 243. doi:10.1007/BF01084401

Abstract

This paper presents a DNA extraction method suitable for fresh, herbarium-stored, lichenized and other fungal specimens. The method is fast and reliable, comparatively inexpensive and is suitable for obtaining PCR amplification quality DNA from large numbers of samples in a short time. The method has been tested with over 300 samples ofAscochyta, Phyllosticta, Ramalina, Parmelia andPhysconia. Amplifiable fungal DNA was extracted from pure cultures, leaf lesions, whole thalli and dissected “only-fungal” sections of lichenized fungi. In addition, the method has proved suitable for use with herbarium specimens of both lichenized and non-lichenized fungi, stored as dried pure cultures or dried infected plant material.

Key words

HerbariumDNA extractionfilamentous fungilichenized fungi

Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Oscar F. Cubero
    • 1
  • Ana Crespo
    • 1
  • Jamshid Fatehi
    • 2
  • Paul D. Bridge
    • 2
  1. 1.Departamento de Biologia Vegetal IIUniversidad ComplutenseMadridSpain
  2. 2.CABI Bioscience UK Centre (Egham)Egham, SurreyUK