Molecular and Cellular Biochemistry

, Volume 151, Issue 1, pp 77–81

Molecular cloning and sequencing of human palmitoyl-CoA ligase and its tissue specific expression

  • Bidyut Ghosh
  • Ernest Barbosa
  • Inderjit Singh
Article

DOI: 10.1007/BF01076899

Cite this article as:
Ghosh, B., Barbosa, E. & Singh, I. Mol Cell Biochem (1995) 151: 77. doi:10.1007/BF01076899

Abstract

A complimentary DNA clone encoding the entire human palmitoyl-CoA ligase has been isolated from a liver cDNA library and sequenced in it's entirety. The predicted product is a 699 amino acid protein. Southern analysis utilizing the human palmitoyl-CoA ligase gene as a probe revealed varying degrees of similarity amongst various mammalian species. The palmitoyl-CoA ligase gene is highly expressed in liver, heart, skeletal muscle and kidney, and to a lesser extent in brain, lung, placenta and pancreas. The expression of palmitoyl-CoA ligase in various tissue parallels the function of this enzyme in the metabolism of fatty acids in these tissues.

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Bidyut Ghosh
    • 1
  • Ernest Barbosa
    • 1
  • Inderjit Singh
    • 1
  1. 1.Department of PediatricsMedical University of South CarolinaCharlestonUSA