Bioprocess Engineering

, Volume 9, Issue 6, pp 245–248

Purification of alkaline protease of Rhizopus oryzae by foam fractionation

Authors

  • Rintu Banerjee
    • Biotechnology unit Department of Chemical EngineeringIndian Institute of Technology Kharagpur
  • Rajeev Agnihotri
    • Biotechnology unit Department of Chemical EngineeringIndian Institute of Technology Kharagpur
  • B. C. Bhattacharyya
    • Biotechnology unit Department of Chemical EngineeringIndian Institute of Technology Kharagpur
Originals

DOI: 10.1007/BF01061529

Cite this article as:
Banerjee, R., Agnihotri, R. & Bhattacharyya, B.C. Bioprocess Engineering (1993) 9: 245. doi:10.1007/BF01061529

Abstract

Studies on the cost effective purification method is very much essential for the industrially important enzyme like protease. As foam fractionation is an important technique in downstream processing of the biologicals, purification of the crude proteolytic enzyme produced by Rhizopus oryzae has been attempted using converging-diverging foam fractionator. The effects of different parameters studied on the purification efficiency by foam fractionation were pH of the broth, air flow rate, initial liquid height. The results obtained were found quite encouraging at pH 10 with 1 LPM of air flow rate at low initial liquid height.

Copyright information

© Springer-Verlag 1993