Journal of Neuro-Oncology

, Volume 20, Issue 1, pp 47–53

Detection of Eber-1 RNA in primary brain lymphomas in immunocompetent and immunocompromised patients


  • R. Bashir
    • Department of Internal MedicineUniversity of Nebraska Medical Center
  • B. McManus
    • Department of Pathology and MicrobiologyUniversity of Nebraska Medical Center
  • C. Cunningham
    • Department of Pathology and MicrobiologyUniversity of Nebraska Medical Center
  • D. Weisenburger
    • Department of Pathology and MicrobiologyUniversity of Nebraska Medical Center
  • F. Hochberg
    • Department of NeurologyMassachusetts General Hospital
Laboratory Investigation

DOI: 10.1007/BF01057960

Cite this article as:
Bashir, R., McManus, B., Cunningham, C. et al. J Neuro-Oncol (1994) 20: 47. doi:10.1007/BF01057960


Tissue from primary central nervous system lymphoma (PCNSL) which developed in five patients with acquired immuno deficiency syndrome (AIDS), nine patients without immunodeficiency, and two Epstein-Barr virus (EBV)-positive control cell lines (B95-8 and Raji) were examined for the presence of EBER-1 RNA. The tissues were hybridized with digoxigenin-labeled sense or anti-sense EBER-1 riboprobes. In all five AIDS-related PCNSLs, strong hybridization signals were found with the EBER-1 anti-sense probe. Signals could be eliminated by preincubation of the tissues with RNase-A. Hybridization with the EBER-1 sense probe showed no signal. All PCNSLs from immunocompetent patients (five paraffin-embedded, four frozen) showed no hybridization signals with EBER-1 sense or antisense probe but good hybridization signals with probes to immunoglobulin kappa or lambda light chain indicating RNA preservation. The paraffin-embedded B95-8-positive control cell-line showed positive hybridization in most cells with the anti-sense EBER-1 probe, and up to one percent of the cells had a weak signal with the sense probe. Most Raji cells showed a uniform signal with the anti-sense EBER-1 probe only. We conclude that, PCNSLs that arise in AIDS patients are associated with latent EBV infections, whereas PCNSLs from immunocompetent patients are not indicating a probable role for EBV in pathogenesis of these tumors.

Key words

lymphomacentral nervous system lymphomaEpstein-Barr virusacquired immuno deficinecy syndromeEBER-1

Copyright information

© Kluwer Academic Publishers 1994