Glycoconjugate Journal

, Volume 5, Issue 4, pp 397–409

A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates

Authors

  • Bruno Domon
    • Mass Spectrometry Facility, Department of ChemistryMassachusetts Institute of Technology
  • Catherine E Costello
    • Mass Spectrometry Facility, Department of ChemistryMassachusetts Institute of Technology
Article

DOI: 10.1007/BF01049915

Cite this article as:
Domon, B. & Costello, C.E. Glycoconjugate J (1988) 5: 397. doi:10.1007/BF01049915

Abstract

A summary of the ion types observed in the Fast Atom Bombardment Mass Spectrometry (FAB-MS) and collision induced decomposition (CID) MS/MS spectra of glycoconjugates (glycosphingolipids, glycopeptides, glycosides and carbohydrates) is presented. The variety of product ion types that arise by cleavages within the carbohydrate moieties has prompted us to introduce a systematic nomenclature to designate these ions. The proposed nomenclature has been developed primarily for FAB-MS, but can be used as well for other ionization techniques [field desorption (FD), direct chemical ionization (DCI), laser desorption/Fourier transform (LD/FT), etc.], and is applicable to spectra recorded in either the positive or negative ion mode during both MS and MS/MS experiments.

Ai, Bi and Ci labels are used to designate fragments containing a terminal (nonreducing end) sugar unit, whereas Xj, Yj and Zj represent ions still containing the aglycone (or the reducing sugar unit). Subscripts indicate the position relative to the termini analogous to the system used in peptides, and superscripts indicate cleavages within carbohydrate rings.

FAB-MS/MS spectra of a native glycosphingolipid and glycopeptide, and a permethylated ganglioside are shown as illustrations.

Key words

glycolipidsglycopeptidesFAB-MStandem mass spectrometry (MS/MS)nomenclature

Abbreviations

FAB-MS

fast atom bombardment mass spectrometry

CID

collision induced decomposition

DCI

direct chemical ionisation

FD

field desorotion

LD/FT

laser desorption/Fourier transform

EI

electron impact

Gm1-ganglioside

II3NeuAc-GgOse4Cer

Fuc-GM1

IV2Fuc,II3NeuAc-GgOse4Cer

hex

hexose

hexNAc

N-acetylhexosamine

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Copyright information

© Glycoconjugate Journal 1988