Biotechnology Letters

, Volume 13, Issue 7, pp 477-482

First online:

Electrophoretic karyotype ofTrichoderma reesei

  • John A. GillyAffiliated withProduct Development Department, Connaught Laboratories, Inc.
  • , Jeffrey A. SandsAffiliated withDepartment of Molecular Biology, Lehigh University

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Pulsed field electrophoresis was used to separate the intact chromosomes of the fungi imperfectiTrichoderma reesei for which no previous karyotype has been available.T. reesei was shown to have six chromosomes with molecular weights ranging from 3.3 to 11.9 megabasepairs (Mbp). The genome size was estimated to be 38 Mbp, indicating thatTrichoderma has a genome larger thanAspergillus nidulans (31 Mbp) but smaller thanNeurospora crassa (47 Mbp).