Fertilizer research

, Volume 6, Issue 3, pp 205–218

The measurement of15N in soil and plant material

Authors

  • G Pruden
    • Soils and Plant Nutrition DepartmentRothamsted Experimental Station
  • DS Powlson
    • Soils and Plant Nutrition DepartmentRothamsted Experimental Station
  • DS Jenkinson
    • Soils and Plant Nutrition DepartmentRothamsted Experimental Station
Article

DOI: 10.1007/BF01048795

Cite this article as:
Pruden, G., Powlson, D. & Jenkinson, D. Fertilizer Research (1985) 6: 205. doi:10.1007/BF01048795

Abstract

A complete procedure for analysing soil and plant samples for total N and atom % excess15N is described. The salicylic acid version of the Kjeldahl method for measuring total N was modified for use in a digestion block, giving quantitative reduction of nitrate in both soil and plant material. Procedures for minimising cross-contamination between samples are specified, including a double-distillation procedure that eliminates ‘memory effects’ when distilling NH3 from Kjeldahl digests. A simple and robust apparatus for converting (NH4)2SO4 to N2 gas for mass spectrometric determination of atom % excess15N is described. The coefficient of variation for replicate measurements of total N in soil and plant material over the range 0.1–2.2% N was 1.0%. The coefficient of variation for measurements of15N in plant material over the range 0.4–2.9 atom % excess15N was 0.2%.

Key words

analytical errorsdetermination of N in plantsdetermination of N in soilsdetermination of15N in plantsdetermination of15N in soilsisotope mass spectrometryKjeldahl method

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985