Biotechnology Letters

, Volume 13, Issue 1, pp 7–12

Enhanced oxygen transfer rates in fermentation using soybean oil-in-water dispersions

  • J. L. Rols
  • G. Goma
Article

DOI: 10.1007/BF01033508

Cite this article as:
Rols, J.L. & Goma, G. Biotechnol Lett (1991) 13: 7. doi:10.1007/BF01033508

Summary

The effect of soybean oil on the volumetric oxygen transfer coefficient during the cultivation ofAerobacter aerogenes cells is presented. For our aeration-agitation conditions (0.278 vvm and 500 rpm), it has been demonstrated that the use 19% (v/v) of soybean oil enabled a 1.85-fold increase of thekl a coefficient (calculated on a per liter aqueous phase basis). For smaller volumetric oil fractions,kL a increased linearly with the oil loading. Because of the oxygen-vector properties of soybean oil, this oil is able to significantly increase thekL a of a bioreactor.

Nomenclature

C*, C

saturation and actual dissolved oxygen concentrations respectively (g/m3)

KLa

volumetric oxygen transfer coefficient (h−1)

KLainitial

kLa measured before the oil addition (h−1)

MO2

molar mass of oxygen (dalton)

N

oxygen transfer rate (g/m3. h)

PO2. PN2

partial pressures ofO2 andN2 in the gas (atm)

PH2OT

partial pressure of water in air at the temperatureT (atm)

PT

total pressure (atm)

Q0

volumetric flow rate of outlet air before seeding (m3/h)

Sp

spreading coefficient (dynes/cm)

T

absolute temperature of outlet gas (K)

Vi

volume of the liquidi in the fermentor (m3)

VM

molar volume at 273 K and 1 atm (m3/mole)

σij

interfacial tension betweeni andj componants (dynes/cm)

v

volumetric fraction of the oil (v/v)

G

gas

O

oil

W

water

i

inlet

o

outlet

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • J. L. Rols
    • 1
  • G. Goma
    • 1
  1. 1.Department de Génie Biochimique et Alimentaire, UA CNRS 544Institut National des Sciences Appliquées, Complexe Scientifique de RangueilToulouse CédexFrance

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