Synthesis of eicosapentaenoyl phosphatidylcholines by polyethylene glycol-modified lipase in benzene
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- Yoshimoto, T., Nakata, M., Yamaguchi, S. et al. Biotechnol Lett (1986) 8: 771. doi:10.1007/BF01020820
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Lipase fromCandida cylindracea was modified with activated polyethylene glycol, which was synthesized from α-carboxymethyl-ω-methoxypoly-(oxyethylene) and N-hydroxysuccinimide with dicyclohexylcarbodiimide. The modified lipase was soluble in organic solvents such as benzene and catalyzed the reaction of ester-exchange to synthesize eicosapentaenoyl phosphatidylcholines from dipalmitoyl phosphatidylcholine and eicosapentaenoic acid. Either one of palmitic acid at C-1 or C-2 position of the phosphatidylcholine was exchanged with eicosapentaenoic acid.