Article

Biotechnology Letters

, Volume 8, Issue 11, pp 771-776

First online:

Synthesis of eicosapentaenoyl phosphatidylcholines by polyethylene glycol-modified lipase in benzene

  • Takayuki YoshimotoAffiliated withLaboratory of Biological Chemistry, Tokyo Institute of Technology
  • , Masahide NakataAffiliated withLaboratory of Biological Chemistry, Tokyo Institute of Technology
  • , Shigehiko YamaguchiAffiliated withLaboratory of Biological Chemistry, Tokyo Institute of Technology
  • , Tadashi FunadaAffiliated withLaboratory of Biological Chemistry, Tokyo Institute of Technology
  • , Yuji SaitoAffiliated withLaboratory of Biological Chemistry, Tokyo Institute of Technology
  • , Yuji InadaAffiliated withLaboratory of Biological Chemistry, Tokyo Institute of Technology

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Summary

Lipase fromCandida cylindracea was modified with activated polyethylene glycol, which was synthesized from α-carboxymethyl-ω-methoxypoly-(oxyethylene) and N-hydroxysuccinimide with dicyclohexylcarbodiimide. The modified lipase was soluble in organic solvents such as benzene and catalyzed the reaction of ester-exchange to synthesize eicosapentaenoyl phosphatidylcholines from dipalmitoyl phosphatidylcholine and eicosapentaenoic acid. Either one of palmitic acid at C-1 or C-2 position of the phosphatidylcholine was exchanged with eicosapentaenoic acid.