Graefe's Archive for Clinical and Experimental Ophthalmology

, Volume 235, Issue 1, pp 27–31

Visualization of retinal and choroidal blood flow with fluorescein leukocyte angiography in rabbits

  • Yunsik Yang
  • Sangduck Kim
  • Jaeduck Kim
Laboratory Investigation

DOI: 10.1007/BF01007834

Cite this article as:
Yang, Y., Kim, S. & Kim, J. Graefe's Arch Clin Exp Ophthalmol (1997) 235: 27. doi:10.1007/BF01007834

Abstract

• Purpose: To visualize the retinal and choroidal leukocytes in rabbits with a new technique, fluorescein leukocyte angiography using a scanning laser ophthalmoscope. • Methods: Blood was withdrawn from an ear vein of a rabbit (New Zealand White), mixed with fluorescein dye in a test tube and centrifuged. The yellow-brown coat layer containing fluorescein-stained leukocytes was collected and injected into the ear vein of the same rabbit while performing fluorescein angiography with a scanning laser ophthalmoscope. The angiographic image displaying circulating fluorescent leukocytes in retinal and choroidal vessels was recorded on a videotape. • Results: Fluorescent leukocytes were clearly visible in the retinal arteries, capillaries, veins and choroidal vessels for more than 1 h. Plugging of leukocytes was seen throughout this period of time in choroidal vessels, while plugging was rare in retinal vessels. • Conclusions: Fluorescein leukocyte angiography is a new technique which can be used for visualization of the leukocytes in retinal and choroidal vessels non-invasively and in vivo.

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Yunsik Yang
    • 1
  • Sangduck Kim
    • 1
  • Jaeduck Kim
    • 1
  1. 1.Department of Ophthalmology, School of MedicineWonkwang UniversityIksan, ChonbukSouth Korea