Planta

, Volume 201, Issue 2, pp 209–218

Growth and ultrastructure ofArabidopsis root hairs: therhd3 mutation alters vacuole enlargement and tip growth

Authors

  • M. E. Galway
    • Department of BiologyThe University of Michigan
  • J. W. HeckmanJr.
    • Center for Electron OpticsMichigan State University
    • Department of BiologyThe University of Michigan
Article

DOI: 10.1007/BF01007706

Cite this article as:
Galway, M.E., Heckman, J.W. & Schiefelbein, J.W. Planta (1997) 201: 209. doi:10.1007/BF01007706

Abstract

The root hairs of plants are tubular projections of root epidermal cells and are suitable for investigating the control of cellular morphogenesis. In wild-typeArabidopsis thaliana (L.) Heynh, growing root hairs were found to exhibit cellular expansion limited to the apical end of the cell, a polarized distribution of organelles in the cytoplasm, and vesicles of several types located near the growing tip. Therhd3 mutant produces short and wavy root hairs with an average volume less than one-third of the wild-type hairs, indicating abnormal cell expansion. The mutant hairs display a striking reduction in vacuole size and a corresponding increase in the relative proportion of cytoplasm throughout hair development. Bead-labeling experiments and ultrastructural analyses indicate that the wavy-hair phenotype of the mutant is caused by asymmetric tip growth, possibly due to abnormally distributed vesicles in cortical areas flanking the hair tips. It is suggested that a major effect of therhd3 mutation is to inhibit vacuole enlargement which normally accompanies root hair cell expansion.

Key words

Arabidopsis (mutant, root hairs)Mutant (Arabidopsis rhd3)Root hair (ultrastructure)Tip growthVacuole
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Copyright information

© Springer-Verlag 1997