The Histochemical Journal

, Volume 21, Issue 2, pp 115–121

Localization of thyroid hormone in subpopulations of rat alveolar macrophages

  • W. K. Liu
  • C. C. Wong
  • N. K. Mak
Papers

DOI: 10.1007/BF01005987

Cite this article as:
Liu, W.K., Wong, C.C. & Mak, N.K. Histochem J (1989) 21: 115. doi:10.1007/BF01005987

Summary

Radioimmunoassay and immunocytochemical staining methods were used to study the distribution of thyroid hormone in rat alveolar macrophages. The cells were fractionated into six subpopulations by Percoll density gradient. Positive immunoreactive tri-iodothyronine (T3) was observed in all subpopulations of macrophages, especially in low-density (1.040 and 1.050 g cm−3) groups, by avidin-biotin-peroxidase immunostaining techniques. The macrophages also showed various patterns of cellular T3 stainability. Results from radioimmunoassay of macrophage extracts also demonstrated that macrophages of low density had a higher level of total T3 than those of higher densities (≥1.060 g cm−3).

Copyright information

© Chapman and Hall Ltd. 1989

Authors and Affiliations

  • W. K. Liu
    • 1
  • C. C. Wong
    • 2
  • N. K. Mak
    • 3
  1. 1.Department of AnatomyThe Chinese University of Hong KongShatin, N.T.Hong Kong
  2. 2.Department of PhysiologyThe Chinese University of Hong KongShatin, N.T.Hong Kong
  3. 3.Department of BiologyHong Kong Baptist CollegeKowloonHong Kong