The Histochemical Journal

, Volume 13, Issue 5, pp 763–779

The effect of different fixatives on chromatin: Cytochemical and ultrastructural approaches

  • A. Fraschini
  • C. Pellicciari
  • M. Biggiogera
  • M. G. Manfredi Romanini
Reviews and Papers

DOI: 10.1007/BF01003288

Cite this article as:
Fraschini, A., Pellicciari, C., Biggiogera, M. et al. Histochem J (1981) 13: 763. doi:10.1007/BF01003288

Summary

This study explores the effects of two types of fixative on chromatin. The first type (acrolein, glutaraldehyde) engenders a high degree of ultrastructural preservation. The other type are fixatives that are widely used in cytochemistry and cytogenetics (acetic acid, 3∶1 by vol. methanol-acetic acid, methanol alone, formaldehyde).

Lymphocytes of adult rats so-fixedin vitro were prepared for electron microscopy or microdensitometric evaluations of smears. Assessments were made of variations in their total protein, nuclear basic protein and DNA contents. DNA was determined both as Feulgen-positive material and by its binding of intercalating dyes (Methyl Green, specific for double-stranded polynucleotides).

Our results showed that some fixatives break up the chromatin organization by acting on particular components of chromatin fibres. They can thus be considered to be destructive agentsin situ. In addition, a revaluation of some aldehyde fixatives is proposed for both ultrastructural and cytochemical research.

Copyright information

© Chapman and Hall Ltd. 1981

Authors and Affiliations

  • A. Fraschini
    • 1
  • C. Pellicciari
    • 1
  • M. Biggiogera
    • 1
  • M. G. Manfredi Romanini
    • 1
  1. 1.Istituto di Istologia, Embriologia e AntropologiaUniversità di Pavia and Centro di Studio per l'Istochimica del CNRPaviaItaly